NEK7 Antibody (YA9739)
(Synonyms: EC 2.7.11.1, NEK7, NEK7_HUMAN, Never in mitosis A-related kinase 7, Never in mitosis gene a-related kinase 7, NIMA (never in mitosis gene a) related kinase 7, NIMA (never in mitosis gene a)-related expressed kinase 7, NimA related protein kinase 7, NIMA-related kinase 7, NimA-related protein kinase 7)NEK7 Antibody (YA9739) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to NEK7.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, FC, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:50-250 | 1:50-800 | 1:50-100 |
Product Details
NEK7 Antibody (YA9739) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to NEK7.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 35 kDa;
Recombinant protein of internal sequence of Human NEK7(aa 200-302).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NEK7 is a Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression. Phosphorylates RPS6KB1 (By similarity). Acts as an essential activator of the NLRP3 inflammasome assembly independently of its kinase activity. Acts by unlocking NLRP3 following NLRP3 tranlocation into the microtubule organizing center (MTOC), relieving NLRP3 autoinhibition and promoting formation of the NLRP3:PYCARD complex, and activation of CASP1. Serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division: interaction with NEK9 prevents interaction with NLRP3 and activation of the inflammasome during mitosis[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Nucleus; Cytoplasm; Cytoplasm, cytoskeleton, spindle pole; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
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Expression
Tissue_Specificity: Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine. -
Isoforms & Post-Translational Modification
NEK7 has 2 isoforms, Q8TDX7-1: amino acid length is 302, molecular weight is 34551 Da (predicted); Q8TDX7-2: amino acid length is 119, molecular weight is 13402 Da (predicted).
Phosphorylation at Ser-195 required for its activation. -
Subunit
Monomer.
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SwissProt ID
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Synonyms
EC 2.7.11.1, NEK7, NEK7_HUMAN, Never in mitosis A-related kinase 7, Never in mitosis gene a-related kinase 7, NIMA (never in mitosis gene a) related kinase 7, NIMA (never in mitosis gene a)-related expressed kinase 7, NimA related protein kinase 7, NIMA-related kinase 7, NimA-related protein kinase 7
Documentation
References
[1]. Yissachar N, et al. Nek7 kinase is enriched at the centrosome, and is required for proper spindle assembly and mitotic progression. FEBS Lett. 2006 Nov 27;580(27):6489-95. [Content Brief]
[2]. Richards MW, et al. An autoinhibitory tyrosine motif in the cell-cycle-regulated Nek7 kinase is released through binding of Nek9. Mol Cell. 2009 Nov 25;36(4):560-70. [Content Brief]
[3]. Adib R, et al. Mitotic phosphorylation by NEK6 and NEK7 reduces the microtubule affinity of EML4 to promote chromosome congression. Sci Signal. 2019 Aug 13;12(594):. [Content Brief]
[4]. Kim S, et al. NEK7 is a centrosomal kinase critical for microtubule nucleation. Biochem Biophys Res Commun. 2007 Aug 17;360(1):56-62. [Content Brief]
[5]. O'Regan L, et al. The Nek6 and Nek7 protein kinases are required for robust mitotic spindle formation and cytokinesis. Mol Cell Biol. 2009 Jul;29(14):3975-90. [Content Brief]
[6]. Haq T, et al. Mechanistic basis of Nek7 activation through Nek9 binding and induced dimerization. Nat Commun. 2015 Nov 2;6:8771. [Content Brief]
[7]. Shi H, et al. NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component. Nat Immunol. 2016 Mar;17(3):250-8. [Content Brief]
[8]. Xiao L, et al. Cryo-EM structures of the active NLRP3 inflammasome disc. Nature. 2023 Jan;613(7944):595-600. [Content Brief]
[9]. Nie L, et al. Consecutive palmitoylation and phosphorylation orchestrates NLRP3 membrane trafficking and inflammasome activation. Mol Cell. 2024 Sep 5;84(17):3336-3353.e7. [Content Brief]
[10]. Sharif H, et al. Structural mechanism for NEK7-licensed activation of NLRP3 inflammasome. Nature. 2019 Jun;570(7761):338-343. [Content Brief]
[11]. Zhang Y, et al. Human cytomegalovirus protein US18 promotes NLRP3 inflammasome activation. Biochem Biophys Res Commun. 2025 Aug 15;775:152110. [Content Brief]
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