COP1 Antibody
(Synonyms: RFWD2, RNF200, COP1, E3 ubiquitin-protein ligase COP1, Constitutive photomorphogenesis protein 1 homolog, RING finger and WD repeat domain protein 2, RING finger protein 200, RING-type E3 ubiquitin transferase RFWD2, hCOP1)COP1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to COP1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-200 |
Product Details
COP1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to COP1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 80 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 80 kDa
Synthetic peptide corresponding to the C-term region of human COP1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
COP1 is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable)
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Subcellular Localization
Nucleus speckle; Cytoplasm
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Expression
Tissue_Specificity: Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.
Induction: By p53/TP53. -
Isoforms & Post-Translational Modification
COP1 has 5 isoforms, Q8NHY2-1: amino acid length is 731, molecular weight is 80474 Da (predicted); Q8NHY2-2: amino acid length is 707, molecular weight is 77691 Da (predicted); Q8NHY2-3: amino acid length is 506, molecular weight is 57527 Da (predicted); Q8NHY2-4: amino acid length is 711, molecular weight is 78066 Da (predicted); Q8NHY2-5: amino acid length is 157, molecular weight is 14914 Da (predicted).发生自泛素化。MTA1 可通过促进其自泛素化使其不稳定COP1 存在 5 个异构体，Q8NHY2-1：氨基酸个数为 731 个，分子量为 80474 Da (预测)；Q8NHY2-2：氨基酸个数为 707 个，分子量为 77691 Da (预测)；Q8NHY2-3：氨基酸个数为 506 个，分子量为 57527 Da (预测)；Q8NHY2-4：氨基酸个数为 711 个，分子量为 78066 Da (预测)；Q8NHY2-5：氨基酸个数为 157 个，分子量为 14914 Da (预测)。Autoubiquitinated. MTA1 destabilizes it by promoting its autoubiquitination
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Subunit
Homodimer.
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SwissProt ID
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Synonyms
RFWD2, RNF200, COP1, E3 ubiquitin-protein ligase COP1, Constitutive photomorphogenesis protein 1 homolog, RING finger and WD repeat domain protein 2, RING finger protein 200, RING-type E3 ubiquitin transferase RFWD2, hCOP1
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