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Cre recombinase Antibody (YA6534)

(Synonyms: Cre antibody; Cyclization recombinase antibody; Recombinase cre antibody; )
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Based on 1 Customer Validation

Cre recombinase Antibody (YA6534) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cre recombinase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IF-Tissue, ICC/IF, FC

  • Reactivity :

    Species independent

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cre recombinase Antibody (YA6534)
  • Cre recombinase Antibody (YA6534) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:10000 1:20000 1:20000 1:1000

Product Details

Description

Cre recombinase Antibody (YA6534) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cre recombinase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Species independent
  • Observed Molecular Weight
    Observed band size: 39 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 39 kDa
Immunogen

Recombinant protein within Cre recombinase aa 1-343 / 343.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719223

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cre recombinase Antibody (YA6534)
    Western blot analysis was performed on protein extracts from Cre recombinase (lane 2, 1 U) and Cre recombinase (lane 3, 2 U) using Cre recombinase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Cre recombinase catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state

  • Isoforms & Post-Translational Modification

    P06956: 343 amino acids, molecular weight 38540 Da.

  • Subunit

    Homotetramer when bound to DNA

  • SwissProt ID

    P06956

  • Gene ID
    2777477 [NCBI]
  • Synonyms

    Cre antibody; Cyclization recombinase antibody; Recombinase cre antibody;

Cre recombinase Antibody (YA6534) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts from Cre recombinase (lane 2, 1 U) and Cre recombinase (lane 3, 2 U) using Cre recombinase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Cre recombinase Antibody (YA6534) Product Image
    1/16
  • Experimental Validation Results for Cre recombinase Antibody (YA6534)
  • Cre recombinase Antibody (YA6534) Product Image
  • Experimental Validation Results for Cre recombinase Antibody (YA6534)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts from Cre recombinase (lane 2, 1 U) and Cre recombinase (lane 3, 2 U) using Cre recombinase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Cre recombinase Antibody (YA6534)
  • Cre recombinase Antibody (YA6534) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts from Cre recombinase (lane 2, 1 U) and Cre recombinase (lane 3, 2 U) using Cre recombinase antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Cre recombinase Antibody (YA6534) Product Image WB