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TRIF Antibody (YA2263)

(Synonyms: TRIF; IIAE6; MyD88-3; PRVTIRB; TICAM-1)
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Based on 1 Customer Validation

TRIF Antibody (YA2263) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRIF.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TRIF Antibody (YA2263)
  • TRIF Antibody (YA2263) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

TRIF Antibody (YA2263) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TRIF.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 98 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 76 kDa
Species Reactivity Database
Immunogen

Recombinant protein of human TRIF

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104624

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TRIF Antibody (YA2263)
    Western blot analysis of extracts from Raji(lane 2(20ug) and Raji(lane 3(40ug) using TRIF Antibody (HY-P82518) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    TRIF is involved in innate immunity against invading pathogens. Adapter used by TLR3, TLR4 (through TICAM2) and TLR5 to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. Phosphorylation by TBK1 on the pLxIS motif leads to recruitment and subsequent activation of the transcription factor IRF3 to induce expression of type I interferon and exert a potent immunity against invading pathogens. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines[1][2][3][4][5][6].

  • Subcellular Localization

    Cytoplasmic vesicle, autophagosome; Cytoplasm, cytosol; Mitochondrion

  • Expression


    Tissue_specificity:Ubiquitously expressed but with higher levels in liver

  • Subunit

    Homodimer (PubMed:12539043). Interacts with AZI2 and IRF7 (PubMed:12471095, PubMed:15611223). Interacts with TICAM2 in TLR4 recruitment (PubMed:12721283, PubMed:25736436).

  • SwissProt ID

    Q8IUC6

  • Gene ID
    148022 [NCBI]
  • Synonyms

    TRIF; IIAE6; MyD88-3; PRVTIRB; TICAM-1

  • Research Field

    Immunology

References

[1]. Yamamoto M, et al. Cutting edge: a novel Toll/IL-1 receptor domain-containing adapter that preferentially activates the IFN-beta promoter in the Toll-like receptor signaling. J Immunol. 2002 Dec 15;169(12):6668-72. [Content Brief]

[2]. Oshiumi H, et al. TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction. Nat Immunol. 2003 Feb;4(2):161-7. [Content Brief]

[3]. Han KJ, et al. Mechanisms of the TRIF-induced interferon-stimulated response element and NF-kappaB activation and apoptosis pathways. J Biol Chem. 2004 Apr 9;279(15):15652-61. [Content Brief]

[4]. Ye W, et al. TRIM8 Negatively Regulates TLR3/4-Mediated Innate Immune Response by Blocking TRIF-TBK1 Interaction. J Immunol. 2017 Sep 1;199(5):1856-1864. [Content Brief]

[5]. Pallett MA, et al. DDX50 Is a Viral Restriction Factor That Enhances IRF3 Activation. Viruses. 2022 Feb 3;14(2):. [Content Brief]

[6]. Liu S, et al. Phosphorylation of innate immune adaptor proteins MAVS, STING, and TRIF induces IRF3 activation. Science. 2015 Mar 13;347(6227):aaa2630. [Content Brief]

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TRIF Antibody (YA2263) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Raji(lane 2(20ug) and Raji(lane 3(40ug) using TRIF Antibody (HY-P82518) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • TRIF Antibody (YA2263) Product Image
    1/16
  • Experimental Validation Results for TRIF Antibody (YA2263)
  • TRIF Antibody (YA2263) Product Image
  • Experimental Validation Results for TRIF Antibody (YA2263)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Raji(lane 2(20ug) and Raji(lane 3(40ug) using TRIF Antibody (HY-P82518) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TRIF Antibody (YA2263)
  • TRIF Antibody (YA2263) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Raji(lane 2(20ug) and Raji(lane 3(40ug) using TRIF Antibody (HY-P82518) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • TRIF Antibody (YA2263) Product Image WB