Caldesmon Antibody (YA1301)
(Synonyms: CALD, CAD, CALD1)Caldesmon Antibody (YA1301) is a Mouse-derived and non-conjugated IgG2b/Kappa monoclonal antibody, targeting to Caldesmon.
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Host:
Mouse
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Isotype:
IgG2b/Kappa
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Application:
WB, IHC-P, IHC-F, IF-Tissue
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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|---|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:100-500 | 1:100-500 | 1:100-500 |
Product Details
Caldesmon Antibody (YA1301) is a Mouse-derived and non-conjugated IgG2b/Kappa monoclonal antibody, targeting to Caldesmon.
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Host Mouse
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Clonality Monoclonal, Recombinant
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 93kDa;
Entrez Gene: 800 Human ; 109624 Mouse ; 25687 Rat
SwissProt: Q05682 Human ; Q62736 Rat
OMIM: 114213 Human
The exact sequence is proprietary to MCE.
Endogenous
affinity purified by Protein G
Non-conjugated
Unmodified
IgG2b/Kappa
Product Properties
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Appearance
Liquid
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Formulation
Supplied in0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Caldesmon is an Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration
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Subcellular Localization
Cytoplasm, cytoskeleton; Cytoplasm, myofibril; Cytoplasm, cytoskeleton, stress fiber
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Expression
Tissue_specificity:High molecular weight calmodulin (isoform 1) is primarily expressed in smooth muscle, while low molecular weight calmodulin (isoforms 2, 3, 4, and 5) are widely distributed in non-muscle tissues and cells. It is not expressed in skeletal muscle or the heart.
Positive sample: NIH/3T3 cell lysates, NIH/3T3, C6, human kidney tissue, mouse kidney tissue, rat kidney tissue. -
Isoforms & Post-Translational Modification
Q05682 has 6 isomers: Q05682-1: 93231 Da (predicted); Q05682-2: 65707 Da (predicted); Q05682-3: 64256 Da (predicted); Q05682-4: 62663 Da (predicted); Q05682-5: 61213 Da (predicted); Q05682-6: 65620 Da (predicted).
In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration (By similarity) -
SwissProt ID
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Synonyms
CALD, CAD, CALD1
Documentation
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