Caldesmon Antibody (YA1301)

Cat. No.: HY-P81556: Data Sheet User Guide for Antibodies
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Caldesmon Antibody (YA1301) is a Mouse-derived and non-conjugated IgG2b/Kappa monoclonal antibody, targeting to Caldesmon.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

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Caldesmon Antibody (YA1301) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명
References

제품 설명

Caldesmon Antibody (YA1301) is a Mouse-derived and non-conjugated IgG2b/Kappa monoclonal antibody, targeting to Caldesmon.

Host

Mouse

Clonality

Monoclonal, Recombinant

분자량

Predicted band size: 93kDa;

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q05682

Gene ID

800 [NCBI]

Immunogen

The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-500

Sensitivity	Endogenous	Purity	affinity purified by Protein G
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2b/Kappa

Appearance

Liquid

Formulation

Supplied in0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity)

Subcellular Localization：Cytoplasm, cytoskeleton; Cytoplasm, myofibril; Cytoplasm, cytoskeleton, stress fiber

Expression：
Tissue_specificity:High molecular weight calmodulin (isoform 1) is primarily expressed in smooth muscle, while low molecular weight calmodulin (isoforms 2, 3, 4, and 5) are widely distributed in non-muscle tissues and cells. It is not expressed in skeletal muscle or the heart.
Positive sample: NIH/3T3 cell lysates, NIH/3T3, C6, human kidney tissue, mouse kidney tissue, rat kidney tissue.

Isoforms & Post-Translational Modification：Q05682 has 6 isomers: Q05682-1: 93231 Da (predicted); Q05682-2: 65707 Da (predicted); Q05682-3: 64256 Da (predicted); Q05682-4: 62663 Da (predicted); Q05682-5: 61213 Da (predicted); Q05682-6: 65620 Da (predicted).
In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration (By similarity)

Database

Entrez Gene: 800 Human ; 109624 Mouse ; 25687 Rat

SwissProt: Q05682 Human ; Q62736 Rat

OMIM: 114213 Human

Synonyms

CALD, CAD, CALD1

각종 서류

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/ovcar-8-cell.html [Content Brief]

Caldesmon Antibody (YA1301) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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