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Adiponectin receptor protein 1 Antibody (YA2700)

(Synonyms: Adiponectin receptor protein 1; Progestin and adipoQ receptor family member I; CGI-45; PAQR1; ADIPOR1)
Customer Review

Based on 1 Customer Validation

Adiponectin receptor protein 1 Antibody (YA2700) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Adiponectin receptor protein 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Adiponectin receptor protein 1 Antibody (YA2700) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

Adiponectin receptor protein 1 Antibody (YA2700) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Adiponectin receptor protein 1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Species Reactivity Database

Entrez Gene: 51094 Human ;

SwissProt: Q96A54 Human ;

Immunogen

A synthesized peptide derived from human ADIPOR1 aa1-46.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105074

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
    Western blot analysis of extracts from Mouse liver tissue (lane 2, 20ug) using Adiponectin receptor protein 1 Antibody (HY-P82955). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Adiponectin receptor protein 1 Antibody (YA2700). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82955, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Adiponectin receptor protein 1 Antibody (YA2700). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82955, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
    Immunocytochemistry analysis of HepG2 cells labeling Adiponectin receptor protein 1 with Adiponectin receptor protein 1 Antibody (HY-P82955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adiponectin receptor protein 1 Antibody ((HY-P82955) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
    Immunocytochemistry analysis of Hela cells labeling Adiponectin receptor protein 1 with Adiponectin receptor protein 1 Antibody (HY-P82955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adiponectin receptor protein 1 Antibody ((HY-P82955) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Adiponectin receptor protein 1 receptor for ADIPOQ, an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. Required for normal glucose and fat homeostasis and for maintaining a normal body weight. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Has high affinity for globular adiponectin and low affinity for full-length adiponectin[1][2].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Widely expressed (PubMed: 16044242) . Highly expressed in the heart and skeletal muscle (PubMed: 12802337) . Moderately expressed in the brain, spleen, kidney, liver, placenta, lung, and peripheral blood leukocytes (PubMed: 12802337) . Weakly expressed in the colon, thymus, and small intestine (PubMed: 12802337) .

  • Subunit

    May form homooligomers and heterooligomers with ADIPOR2 (PubMed:12802337).

  • SwissProt ID

    Q96A54

  • Gene ID
    51094 [NCBI]
  • Synonyms

    Adiponectin receptor protein 1; Progestin and adipoQ receptor family member I; CGI-45; PAQR1; ADIPOR1

  • Research Field

    Cardiovascular

Adiponectin receptor protein 1 Antibody (YA2700) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Mouse liver tissue (lane 2, 20ug) using Adiponectin receptor protein 1 Antibody (HY-P82955). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Adiponectin receptor protein 1 Antibody (YA2700). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82955, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Adiponectin receptor protein 1 Antibody (YA2700). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82955, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Adiponectin receptor protein 1 with Adiponectin receptor protein 1 Antibody (HY-P82955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adiponectin receptor protein 1 Antibody ((HY-P82955) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Adiponectin receptor protein 1 with Adiponectin receptor protein 1 Antibody (HY-P82955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adiponectin receptor protein 1 Antibody ((HY-P82955) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Adiponectin receptor protein 1 Antibody (YA2700) Product Image
    1/16
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Adiponectin receptor protein 1 Antibody (YA2700) Product Image
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Mouse liver tissue (lane 2, 20ug) using Adiponectin receptor protein 1 Antibody (HY-P82955). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Adiponectin receptor protein 1 Antibody (YA2700). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82955, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Adiponectin receptor protein 1 Antibody (YA2700). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82955, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Adiponectin receptor protein 1 with Adiponectin receptor protein 1 Antibody (HY-P82955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adiponectin receptor protein 1 Antibody ((HY-P82955) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Adiponectin receptor protein 1 with Adiponectin receptor protein 1 Antibody (HY-P82955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adiponectin receptor protein 1 Antibody ((HY-P82955) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Experimental Validation Results for Adiponectin receptor protein 1 Antibody (YA2700)
  • Adiponectin receptor protein 1 Antibody (YA2700) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Mouse liver tissue (lane 2, 20ug) using Adiponectin receptor protein 1 Antibody (HY-P82955). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Adiponectin receptor protein 1 Antibody (YA2700) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Adiponectin receptor protein 1 Antibody (YA2700). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82955, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Adiponectin receptor protein 1 Antibody (YA2700) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue using Adiponectin receptor protein 1 Antibody (YA2700). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P82955, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Adiponectin receptor protein 1 Antibody (YA2700) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Adiponectin receptor protein 1 with Adiponectin receptor protein 1 Antibody (HY-P82955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adiponectin receptor protein 1 Antibody ((HY-P82955) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Adiponectin receptor protein 1 Antibody (YA2700) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Adiponectin receptor protein 1 with Adiponectin receptor protein 1 Antibody (HY-P82955) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Adiponectin receptor protein 1 Antibody ((HY-P82955) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Adiponectin receptor protein 1 Antibody (YA2700) Product Image WB
  • Adiponectin receptor protein 1 Antibody (YA2700) Product Image IHC-P
  • Adiponectin receptor protein 1 Antibody (YA2700) Product Image IHC-P
  • Adiponectin receptor protein 1 Antibody (YA2700) Product Image ICC
  • Adiponectin receptor protein 1 Antibody (YA2700) Product Image ICC