SLFN12 Antibody (YA5976)
(Synonyms: SLFN12; Ribonuclease SLFN12 ; Schlafen family member 12; )
Based on 1 Customer Validation
SLFN12 Antibody (YA5976) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLFN12.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, FC
-
Reactivity :
Human
-
Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
FC
FC: Flow Cytometry
|
|---|---|---|
| Dilution Ratio | 1:1000-5000 | 1:100-300 |
Product Details
SLFN12 Antibody (YA5976) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SLFN12.
-
Host Rabbit
-
Clonality Monoclonal
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 64 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
-
Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Verification Images
-
Western blot analysis of extracts from K562 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、HEK293 (lane 3(20μg)) and SH-SY5Y (lane 4(20μg)) using SLFN12 Antibody (HY-P86284) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Background
-
Function
SLFN12 is a Ribonuclease which is part of an E2/17beta-estradiol-induced pro-apoptotic signaling pathway. E2 stabilizes the PDE3A/SLFN12 complex in the cytosol, promoting the dephosphorylation of SLFN12 and activating its pro-apoptotic ribosomal RNA/rRNA ribonuclease activity. This apoptotic pathway might be relevant in tissues with high concentration of E2 and be for instance involved in placenta remodeling. May play a role in cell differentiation[1][2][3][4][5].
-
Subcellular Localization
Nucleus; Cytoplasm, cytosol
-
Subunit
Homodimer (PubMed:34272366). Interacts with PDE3A; direct low affinity interaction which is stimulated by binding of 17beta-estradiol/E2 to PDE3A and that positively regulates the ribonuclease activity of SLFN12 (PubMed:31420216, PubMed:34272366, PubMed:34707099, PubMed:35104454). Interacts with SERPINB12; as part of a pathway regulating cell differentiation (PubMed:30045019)
-
SwissProt ID
-
Synonyms
SLFN12; Ribonuclease SLFN12 ; Schlafen family member 12;
Documentation
Powered by Bioz
