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Cytochrome P450 24A1 Antibody (YA2121)

(Synonyms: CYP24A1; CYP24; 1; 25-dihydroxyvitamin D(3) 24-hydroxylase; mitochondrial; 24-OHase; Vitamin D(3) 24-hydroxylase; Cytochrome P450 24A1; Cytochrome P450-CC24)
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Based on 1 Customer Validation

Cytochrome P450 24A1 Antibody (YA2121) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytochrome P450 24A1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cytochrome P450 24A1 Antibody (YA2121)
  • Cytochrome P450 24A1 Antibody (YA2121) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Cytochrome P450 24A1 Antibody (YA2121) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytochrome P450 24A1.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 54-59 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 59 kDa
Species Reactivity Database

Entrez Gene: 1591 Human

SwissProt: Q07973 Human

Immunogen

A synthesized peptide derived from human CYP24A1 aa100-150/514.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cytochrome P450 24A1 Antibody (YA2121)
    Western blot analysis of extracts from HepG2 (lane2, 20μg) and DU145 (lane3, 20μg) using Cytochrome P450 24A1 Antibody (HY-P82376). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Cytochrome P450 24A1 is an A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D(3)) and the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)). With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid. With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25(OH)D3-26,23-lactone as end product. Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S-hydroxycholecalciferol and 20S,23-dihydroxycholecalciferol. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin[1][2][3][4][5][6][7].

  • Subcellular Localization

    Mitochondrion

  • Isoforms & Post-Translational Modification

    Q07973 has 3 isomers: Q07973-1: 58875 Da (predicted); Q07973-2: 51207 Da (predicted); Q07973-3: 43051 Da (predicted).

  • SwissProt ID

    Q07973

  • Gene ID
    1591 [NCBI]
  • Synonyms

    CYP24A1; CYP24; 1; 25-dihydroxyvitamin D(3) 24-hydroxylase; mitochondrial; 24-OHase; Vitamin D(3) 24-hydroxylase; Cytochrome P450 24A1; Cytochrome P450-CC24

  • Research Field

    Cardiovascular

Cytochrome P450 24A1 Antibody (YA2121) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane2, 20μg) and DU145 (lane3, 20μg) using Cytochrome P450 24A1 Antibody (HY-P82376). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Cytochrome P450 24A1 Antibody (YA2121) Product Image
    1/16
  • Experimental Validation Results for Cytochrome P450 24A1 Antibody (YA2121)
  • Cytochrome P450 24A1 Antibody (YA2121) Product Image
  • Experimental Validation Results for Cytochrome P450 24A1 Antibody (YA2121)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane2, 20μg) and DU145 (lane3, 20μg) using Cytochrome P450 24A1 Antibody (HY-P82376). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cytochrome P450 24A1 Antibody (YA2121)
  • Cytochrome P450 24A1 Antibody (YA2121) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane2, 20μg) and DU145 (lane3, 20μg) using Cytochrome P450 24A1 Antibody (HY-P82376). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Cytochrome P450 24A1 Antibody (YA2121) Product Image WB