PIWIL1 Antibody
(Synonyms: MIWI, PIWIL1)PIWIL1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to PIWIL1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS with 0.02% sodium azide,50% glycerol,pH7.3.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50-200 | 1:50-200 |
Product Details
PIWIL1 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to PIWIL1.
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Host Rabbit
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Clonality polyclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 99kDa;
Recombinant protein of human PIWIL1
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS with 0.02% sodium azide,50% glycerol,pH7.3.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PIWIL1 is an Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Required to sequester RNF8 in the cytoplasm until late spermatogenesis; RNF8 being released upon ubiquitination and degradation of PIWIL1[1][2][3][4][5][6][7].
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Subcellular Localization
Cytoplasm
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Expression
Tissue_Specificity: Expressed in brain. Expressed in testis, specifically in spermatocytes (at protein level). Only detected in germ lineage cells of adult testis. Expressed in male gonads 2 weeks after birth at the initiation of spermatogenesis, but not expressed in female gonads.
Induction: Expression is activated by MYBL1/A-MYB. -
Isoforms & Post-Translational Modification
PIWIL1 has 2 isoforms, Q9JMB7-1: amino acid length is 862, molecular weight is 98574 Da (predicted); Q9JMB7-2: amino acid length is 835, molecular weight is 95484 Da (predicted).
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in late spermatids, leading to its degradation. Ubiquitination only takes place following piRNA-binding in adult testis. Ubiquitination and degradation in late spermatogenesis by APC/C is probably required to release RNF8 from the cytoplasm and promote histone to protamine exchange by RNF8. -
Subunit
Interacts (via Piwi domain) with DICER1, suggesting that it forms ribonucleoprotein RISC complexes; this interaction is regulated by HSP90AB1 activity.
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SwissProt ID
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Synonyms
MIWI, PIWIL1
Documentation
[1]. Kuramochi-Miyagawa S, et al. Two mouse piwi-related genes: miwi and mili. Mech Dev. 2001 Oct;108(1-2):121-33. [Content Brief]
[2]. Simon B, et al. Recognition of 2'-O-methylated 3'-end of piRNA by the PAZ domain of a Piwi protein. Structure. 2011 Feb 9;19(2):172-80. [Content Brief]
[3]. Reuter M, et al. Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing. Nature. 2011 Nov 27;480(7376):264-7. [Content Brief]
[4]. Cora E, et al. The MID-PIWI module of Piwi proteins specifies nucleotide- and strand-biases of piRNAs. RNA. 2014 Jun;20(6):773-81. [Content Brief]
[5]. Grivna ST, et al. MIWI associates with translational machinery and PIWI-interacting RNAs (piRNAs) in regulating spermatogenesis. Proc Natl Acad Sci U S A. 2006 Sep 5;103(36):13415-20. [Content Brief]
[6]. Kotaja N, et al. Interplay of PIWI/Argonaute protein MIWI and kinesin KIF17b in chromatoid bodies of male germ cells. J Cell Sci. 2006 Jul 1;119(Pt 13):2819-25. [Content Brief]
[7]. Gou LT, et al. Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis. Cell. 2017 Jun 1;169(6):1090-1104.e13. [Content Brief]
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