ABCB5 Antibody (YA836)
(Synonyms: ABCB5 P-gp; ATP binding cassette sub family B (MDR/TAP) member 5; P glycoprotein ABCB5)
Based on 1 Customer Validation
ABCB5 Antibody (YA836) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ABCB5.
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Host:
Mouse
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Isotype:
IgG1
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Application:
WB, ICC/IF
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Reactivity :
Human
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Formulation:
Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Antibody Dilution Buffer (for Western Blot)
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Ultra High Sensitivity ECL Kit
HY-K1005
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Hematoxylin-Eosin Staining Kit
HY-K0315
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3-Color Prestained Protein Marker (10-190 kDa)
HY-K1011
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High Sensitivity ECL Kit
HY-K2005
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Penicillin-Streptomycin (100×), Sterile
HY-K1006
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DPBS Buffer (1×)
HY-K3008
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GelHP Protein Gel (Tris-Gly, 10%, 15 well)
HY-K1112
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Ponceau Staining Solution (Ready-to-use, 1×)
HY-K1109
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GelHP Protein Gel (Tris-Gly, 12%, 10 well)
HY-K1113
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GelHP Protein Gel (Tris-Gly, 10%, 10 well)
HY-K1111
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
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| Dilution Ratio | 1:500-1:1000 | 1:50-1:200 |
Product Details
ABCB5 Antibody (YA836) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ABCB5.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 90 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 139 kDa
Synthetic peptide corresponding to Human ABCB5.The exact sequence is proprietary to MCE.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG1
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from A431(lane 2(20ug) and A431(lane 3(40ug) using ABCB5 Antibody (HY-P80527) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A549 cells labeling ABCB5 with ABCB5 Antibody (HY-P80527) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ABCB5 Antibody (HY-P80527) at 1/100dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). -
Immunocytochemistry analysis of A549 cells labeling ABCB5 with ABCB5 Antibody (HY-P80527) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with ABCB5 Antibody (HY-P80527) at 1/50dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Background
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Function
Energy-dependent efflux transporter responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:12960149, PubMed:15205344, PubMed:15899824, PubMed:22306008). Specifically present in limbal stem cells, where it plays a key role in corneal development and repair (By similarity)
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Subcellular Localization
Cell membrane; Multi-pass membrane protein
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Expression
Tissue_specificity:In epidermal melanocytes, CD133-expressing progenitor cells express this protein (protein level) . This protein is widely expressed and specifically expressed in pigment cells. It is highly expressed in various malignant tissues: highly expressed in clinical melanoma and lowly expressed in normal skin. In melanoma, this protein marks malignant melanoma initiating cells (MMICs) , which have unlimited self-renewal capacity, leading to irreversible tumor progression and metastasis, thus triggering clinical malignancy. This protein is also highly expressed in various leukemia cells. It is also expressed in the basal limbal epithelium. -
Isoforms & Post-Translational Modification
Q2M3G0 has 4 isomers: Q2M3G0-4: 138641 Da (predicted); Q2M3G0-2: 14691 Da (predicted); Q2M3G0-3: 14081 Da (predicted); Q2M3G0-1: 89832 Da (predicted).
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SwissProt ID
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Synonyms
ABCB5 P-gp; ATP binding cassette sub family B (MDR/TAP) member 5; P glycoprotein ABCB5
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Research Field
Signal Transduction
Documentation
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Data Sheet (259 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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User Guide for Antibodies (1077 KB)
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