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SIRT3 Antibody (YA080)

(Synonyms: SIR2L3)
Customer Review

Based on 1 Customer Validation

SIRT3 Antibody (YA080) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SIRT3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SIRT3 Antibody (YA080)
  • Experimental Validation Results for SIRT3 Antibody (YA080)
  • SIRT3 Antibody (YA080) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

SIRT3 Antibody (YA080) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SIRT3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 44 kDa
Species Reactivity Database

Entrez Gene: 23410 Human

SwissProt: Q9NTG7 Human

Immunogen

Synthetic peptide corresponding to Human SIRT3.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102247

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SIRT3 Antibody (YA080)
    Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), HT-29 (lane 3, 15 μg), and A549 (lane 4, 15 μg) using SIRT3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for SIRT3 Antibody (YA080)
    Western blot analysis of extracts from 293T (lane 1) and Hela (lane 2) using SIRT3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    SIRT3 is the major mitochondrial protein lysine deacetylase and regulates mitochondrial homeostasis by deacetylating proteins involved in energy metabolism, antioxidant defense, and redox adaptation[1][2]. Mechanistically, SIRT3 limits mitochondrial oxidative damage through targets including SOD2, PRDX3, COX4I2, and ALDH2, thereby linking lysine acetylation to reactive oxygen species control, respiratory-chain function, and aldehyde detoxification[3][4][5][6]. In disease models, SIRT3 deletion promotes endothelial dysfunction, vascular inflammation, vascular hypertrophy, and age-dependent hypertension, whereas SIRT3 overexpression reduces vascular oxidative stress and attenuates angiotensin II- and deoxycorticosterone acetate-salt-induced hypertension[3]. In intestinal ischemia/reperfusion injury, SIRT3-mediated PRDX3 deacetylation reduces mitochondrial oxidative damage and apoptosis; in osteoarthritis models, SIRT3 deacetylates COX4I2 to maintain mitochondrial respiratory-chain homeostasis and protect cartilage integrity[4][5]. Compared with related isoforms, SIRT3 differs experimentally because it localizes to mitochondria, whereas structurally similar SIRT1 and SIRT2 localize primarily to the nucleus and cytosol[1]. For experimental applications, honokiol activates SIRT3 in osteoarthritis models, while mitochondria-targeted SIRT3 inhibitors increase MnSOD acetylation with limited effects on known SIRT1 and SIRT2 targets[5][1].

  • Subcellular Localization

    Mitochondrion matrix

  • Expression


    Tissue_specificity:Broad expression

  • Isoforms & Post-Translational Modification

    Q9NTG7 has 2 isomers: Q9NTG7-1: 43573 Da (predicted); Q9NTG7-2: 28567 Da (predicted).
    Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity

  • Subunit

    Upon metabolic stress, forms a complex composed of FOXO3, SIRT3 and mitochondrial RNA polymerase POLRMT; the complex is recruited to mtDNA in a SIRT3-dependent manner (PubMed:23283301). Also forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT (PubMed:29445193). Interacts with NDUFA9, ACSS1, IDH2 and GDH (PubMed:16788062, PubMed:18680753, PubMed:18794531, PubMed:19535340). Interacts with PCCA (PubMed:23438705)

  • SwissProt ID

    Q9NTG7

  • Gene ID
    23410 [NCBI]
  • Synonyms

    SIR2L3

  • Research Field

    Epigenetics and Nuclear Signaling

[1]. Troelsen KS, Bæk M, Nielsen AL, Madsen AS, Rajabi N, Olsen CA. Mitochondria-targeted inhibitors of the human SIRT3 lysine deacetylase. RSC Chem Biol. 2021 Feb 1;2(2):627-635. [Content Brief]

[2]. Sack MN. The role of SIRT3 in mitochondrial homeostasis and cardiac adaptation to hypertrophy and aging. J Mol Cell Cardiol. 2012 Mar;52(3):520-5. doi: 10.1016/j.yjmcc.2011.11.004. Epub 2011 Nov 19. PMID: 22119802; PMCID: PMC3294048. et al. The role of SIRT3 in mitochondrial homeostasis and cardiac adaptation to hypertrophy and aging. J Mol Cell Cardiol. 2012 Mar;52(3):520-5. [Content Brief]

[3]. Dikalova AE, et al. Mitochondrial Deacetylase Sirt3 Reduces Vascular Dysfunction and Hypertension While Sirt3 Depletion in Essential Hypertension Is Linked to Vascular Inflammation and Oxidative Stress. Circ Res. 2020 Feb 14;126(4):439-452. [Content Brief]

[4]. Wang Z, et al. SIRT3-mediated deacetylation of PRDX3 alleviates mitochondrial oxidative damage and apoptosis induced by intestinal ischemia/reperfusion injury. Redox Biol. 2020 Jan;28:101343. [Content Brief]

[5]. Zhang Y, et al. Reprogramming of Mitochondrial Respiratory Chain Complex by Targeting SIRT3-COX4I2 Axis Attenuates Osteoarthritis Progression. Adv Sci (Weinh). 2023 Apr;10(10):e2206144. [Content Brief]

[6]. Harris PS, et al. Characterizing Sirtuin 3 Deacetylase Affinity for Aldehyde Dehydrogenase 2. Chem Res Toxicol. 2017 Mar 20;30(3):785-793. [Content Brief]

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SIRT3 Antibody (YA080) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), HT-29 (lane 3, 15 μg), and A549 (lane 4, 15 μg) using SIRT3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from 293T (lane 1) and Hela (lane 2) using SIRT3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • SIRT3 Antibody (YA080) Product Image
    1/16
  • Experimental Validation Results for SIRT3 Antibody (YA080)
  • Experimental Validation Results for SIRT3 Antibody (YA080)
  • SIRT3 Antibody (YA080) Product Image
  • Experimental Validation Results for SIRT3 Antibody (YA080)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), HT-29 (lane 3, 15 μg), and A549 (lane 4, 15 μg) using SIRT3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for SIRT3 Antibody (YA080)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from 293T (lane 1) and Hela (lane 2) using SIRT3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for SIRT3 Antibody (YA080)
  • Experimental Validation Results for SIRT3 Antibody (YA080)
  • SIRT3 Antibody (YA080) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from 293 (lane 1, 15 μg), HepG2 (lane 2, 15 μg), HT-29 (lane 3, 15 μg), and A549 (lane 4, 15 μg) using SIRT3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • SIRT3 Antibody (YA080) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from 293T (lane 1) and Hela (lane 2) using SIRT3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • SIRT3 Antibody (YA080) Product Image WB
  • SIRT3 Antibody (YA080) Product Image WB