Caspase-12 Antibody (YA3489)

Cat. No.: HY-P81095: Ficha de datos COA
SDS
User Guide for Antibodies
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Technical Support

Caspase-12 Antibody (YA3489) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-12.

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Tamaño	Stock	Cantidad
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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Caspase-12 Antibody (YA3489) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn

Descripciòn

Caspase-12 Antibody (YA3489) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Caspase-12.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Peso molecular

Predicted band size: 48 kDa;

Observed band size: 53 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O08736

Gene ID

100506742 [NCBI]

Immunogen

Recombinant protein within mouse Caspase-12 aa 101-419.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000

Sensitivity	Endogenous	Pureza	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

ALL WB IHC-P

Western blot analysis of extracts from C2C12 (lane 2(20μg) and C2C12 (lane 3(40μg) using Caspase-12 (HY-P81095) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat heart tissue using Caspase-12 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat heart tissue using Caspase-12 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Involved in the activation cascade of caspases responsible for apoptosis execution

Expression：
Tissue_specificity:Mainly expressed in skeletal muscle and lung
Positive sample: C2C12 cell lysate, J774A.1 cell lysate, human heart tissue, mouse heart tissue, rat heart tissue.

Subunit：Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by two subunits (Potential). Interacts with TRAF2 under resting conditions; this interaction is reduced in ER stress conditions

RRID

AB_3103206

Database

Entrez Gene: 100506742 Human ; 12364 Mouse ; 156117 Rat

SwissProt: Q6UXS9 Human ; O08736 Mouse ; Q920D5 Rat

OMIM: 608633 Human

Synonyms

CASP 12 antibody; CASP-12 antibody; Casp12 antibody; CASP12P1 antibody; caspase 12 (gene/pseudogene) antibody; caspase 12 pseudogene 1 antibody; CASPC_HUMAN antibody; Inactive caspase-12 antibody; OTTHUMP00000207032 antibody;

Documentación

Select Batch:

Ficha de datos (231 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Caspase-12 Antibody (YA3489) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nombre del producto:: Caspase-12 Antibody (YA3489)
Cat. No.:: HY-P81095
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1/16

MedchemExpress Validation
Western blot analysis of extracts from C2C12 (lane 2(20μg) and C2C12 (lane 3(40μg) using Caspase-12 (HY-P81095) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
1/16

MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded rat heart tissue using Caspase-12 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
1/16

MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded rat heart tissue using Caspase-12 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
1/16

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Caspase-12 Antibody (YA3489)

Caspase-12 Antibody (YA3489) Recomendaciones destacadas:

Product Detail

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Background

Descripciòn

Caspase-12 Antibody (YA3489) Related Classifications

Productos vistos recientemente:

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