Myosin light chain kinase/MLCK Antibody (YA3133)

Cat. No.: HY-P83388: Fiche technique COA
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Myosin light chain kinase/MLCK Antibody (YA3133) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin light chain kinase/MLCK.

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Size	Stock	Quantité
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

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Myosin light chain kinase/MLCK Antibody (YA3133) Recommandations en vedette:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Description

Description

Myosin light chain kinase/MLCK Antibody (YA3133) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin light chain kinase/MLCK.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Masse moléculaire

Predicted band size: 211 kDa;

Observed band size: 130-140 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q15746

Gene ID

85366 [NCBI]

Immunogen

A synthesized peptide derived from human Myosin light chain kinase aa1850-1914.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Pureté	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Verification Image

ICC

Immunocytochemistry analysis of NIH-3T3 cells labeling MYLK with MYLK antibody (HY-P83388) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MYLK antibody (HY-P83388) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of HeLa cells labeling MYLK with MYLK antibody (HY-P83388) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with MYLK antibody (HY-P83388) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction tH2O2gh a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA-dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury; triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca(2+) entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells. Responsible for high proliferative ability of breast cancer cells tH2O2gh anti-apoptosis

Subcellular Localization：Cytoplasm; Cell projection, lamellipodium; Cleavage furrow; Cytoplasm, cytoskeleton, stress fiber

Expression：
Tissue_specificity:Smooth muscle and non-muscle isoenzymes are expressed in various human tissues and cultured endothelial cells, and their qualitative expression appears to be neither tissue-specific nor developmentally specific. Non-muscle isoenzyme 2 is a major splice variant expressed in various tissues. Telokin is present in various human tissues. Upon stimulation by tumor necrosis factor (TNF) , Telokin accumulates in well-differentiated intestinal epithelial cells.

Induction:Induced by tumor necrosis factor (TNF) . Repressed by androgens (e.g. R1881)

Isoforms & Post-Translational Modification：Q15746 has 11 isomers: Q15746-1: 210715 Da (predicted); Q15746-2: 203055 Da (predicted); Q15746-3: 205059 Da (predicted); Q15746-4: 197399 Da (predicted); Q15746-5: 202350 Da (predicted); Q15746-6: 210586 Da (predicted); Q15746-7: 110205 Da (predicted); Q15746-8: 16970 Da (predicted); Q15746-9: 80410 Da (predicted); Q15746-10: 16840 Da (predicted); Q15746-11: 110076 Da (predicted).
Can probably be down-regulated by phosphorylation. Tyrosine phosphorylation by ABL1 increases kinase activity, reverses MLCK-mediated inhibition of Arp2/3-mediated actin polymerization, and enhances CTTN-binding. Phosphorylation by SRC at Tyr-464 and Tyr-471 promotes CTTN binding;The C-terminus is deglutamylated by AGTPBP1/CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown (By similarity);Acetylated at Lys-608 by NAA10/ARD1 via a calcium-dependent signaling; this acetylation represses kinase activity and reduces tumor cell migration

Subunit：All isoforms including Telokin bind calmodulin. Interacts with SVIL (By similarity). Interacts with CTTN; this interaction is reduced during tH2O2bin-induced endothelial cell (EC) contraction but is promoted by the barrier-protective agonist sphingosine 1-phosphate (S1P) within lamellipodia. A complex made of ABL1, CTTN and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Binds to NAA10/ARD1 and PTK2B/PYK2

RRID

AB_3105501

Database

Entrez Gene: 85366 Human ; 228785 Mouse ; 117558 Rat

SwissProt: Q15746 Human ; Q8VCR8 Mouse ; P20689 Rat

OMIM: 613780 Human

Research Field

Signal Transduction

Synonyms

KMLS; KRP; MLCK; MYLK; myosin light chain kinase; smooth muscle and non-muscle isozymes; myosin light polypeptide kinase; telokin

Documentation

Select Batch:

Fiche technique (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Myosin light chain kinase/MLCK Antibody (YA3133) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Myosin light chain kinase/MLCK Antibody (YA3133)

Myosin light chain kinase/MLCK Antibody (YA3133) Recommandations en vedette:

Product Detail

Verification Image

Background

Description

Myosin light chain kinase/MLCK Antibody (YA3133) Related Classifications

Vos produits récemment consultés:

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