Monocarboxylic Acid Transporter 1 Antibody (YA3244)(PBS only)

Cat. No.: HY-P83499A: Data Sheet User Guide for Antibodies
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Monocarboxylic Acid Transporter 1 Antibody (YA3244) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Monocarboxylic Acid Transporter 1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Monocarboxylic Acid Transporter 1 Antibody (YA3244) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Monocarboxylic Acid Transporter 1.

Host

Rabbit

Molecular Weight

Predicted band size: 54 kDa;

Observed band size: 54 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P53985

Gene ID

6566 [NCBI]

Immunogen

A synthetic peptide of human Monocarboxylic acid transporter 1

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Bidirectional proton-coupled monocarboxylate transporter (PubMed:12946269, PubMed:32946811, PubMed:33333023). Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, acetate and the ketone bodies acetoacetate and beta-hydroxybutyrate, and thus contributes to the maintenance of intracellular pH (PubMed:12946269, PubMed:33333023). The transport direction is determined by the proton motive force and the concentration gradient of the substrate monocarboxylate. MCT1 is a major lactate exporter (By similarity). Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis (By similarity). Facilitates the protonated monocarboxylate form of succinate export, that its transient protonation upon muscle cell acidification in exercising muscle and ischemic heart (PubMed:32946811). Functions via alternate outward- and inward-open conformation states. Protonation and deprotonation of 309-Asp is essential for the conformational transition (PubMed:33333023)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein; Apical cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Widely expressed (PubMed:12115955, PubMed:15505343, PubMed:15901598) . Detected in heart and in blood lymphocytes and monocytes (at protein level) (PubMed:15505343)

Isoforms & Post-Translational Modification：P53985 has 2 isomers: P53985-1: 53944 Da (predicted); P53985-2: 46234 Da (predicted).

Subunit：Interacts with EMB; interaction mediates SLC16A1 targeting to the plasma membrane (By similarity). Interacts with isoform 2 of BSG; interaction mediates SLC16A1 targeting to the plasma membrane (PubMed:17127621, PubMed:25957687, PubMed:33333023)

Research Field

Cancer

Synonyms

HHF7; MCT 1; MCT; Slc16a1

Documentation

User Guide for Antibodies (1077 KB)