CDC34 Antibody (YA7693)

Cat. No.: HY-P88008: Data Sheet User Guide for Antibodies
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CDC34 Antibody (YA7693) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDC34.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

CDC34 Antibody (YA7693) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDC34.

Host

Rabbit

Clonality

Recombinant

Molecular Weight

Predicted band size: 27 kDa;

Observed band size: 35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse Predicted Reactivity: Rat

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P49427

Immunogen

A synthesized peptide derived from human CDC34

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
FC FC: Flow Cytometry	1:50-100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200
IP IP: Immunoprecipitation	1:20-50

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：E2 ubiquitin-conjugating enzyme that accepts ubiquitin from an E1 ubiquitin-activating protein, and catalyzes its covalent attachment to other proteins by an E3 ubiquitin-protein ligase complex (PubMed:10329681, PubMed:17588522, PubMed:20061386, PubMed:38326650). In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:22496338). Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation (PubMed:10329681, PubMed:10918611, PubMed:17698585). Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1 (PubMed:10871850, PubMed:15652359, PubMed:19112177). Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation (PubMed:19126550). Also involved in the degradation of beta-catenin (PubMed:12037680). Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes (PubMed:11805320, PubMed:12060736)

Subcellular Localization：Cytoplasm,Nucleus

Expression：
Tissue_Specificity: Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription
Induction: Negatively regulated by the let-7 microRNA

Isoforms & Post-Translational Modification：P49427: 236 amino acids, molecular weight 26737 Da.

Subunit：Interacts with multiple Cul1-RING E3 ubiquitin-protein ligase complexes, also known as SCF (SKP1-CUL1-F-box protein) complexes (PubMed:10918611, PubMed:11675391, PubMed:18851830, PubMed:19112177, PubMed:19945379, PubMed:24316736)

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/baf3-cell.html [Content Brief]