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SUMO Conjugating Enzyme UBC9 Antibody (YA3301)

(Synonyms: UBE2I; UBC9; UBCE9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18)
Customer Review

Based on 1 Customer Validation

SUMO Conjugating Enzyme UBC9 Antibody (YA3301) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SUMO Conjugating Enzyme UBC9.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC ICC
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image
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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:5000 1:100-1:200 1:50-1:100 1:50-1:200 1:20-1:50 1:20

Product Details

Description

SUMO Conjugating Enzyme UBC9 Antibody (YA3301) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SUMO Conjugating Enzyme UBC9.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 18 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 18 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human UBE2I/UBC9

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    Western blot analysis of extracts from Hela(lane2(20μg), A549(20μg), A375(lane4(20μg) and HepG2(lane5(20μg) using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    Immunohistochemical analysis of paraffin-embedded human rectum tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    Immunohistochemical analysis of paraffin-embedded human stomach tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    Immunohistochemical analysis of paraffin-embedded human duodenum tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    Flow cytometric analysis of 1X106 HeLa cells labeling SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    Immunocytochemistry analysis of HeLa cells labeling SUMO Conjugating Enzyme UBC9 with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    Immunocytochemistry analysis of HepG2 cells labeling SUMO Conjugating Enzyme UBC9 with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    SUMO Conjugating Enzyme UBC9 is an Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity[1].

  • Subcellular Localization

    Nucleus; Cytoplasm; Cytoplasm, perinuclear region

  • Expression


    Tissue_specificity:It is expressed in the heart, skeletal muscle, pancreas, kidneys, liver, lungs, placenta, and brain. It is also expressed in the testes and thymus.

  • Subunit

    Forms a complex with SENP6 and UBE2I in response to UV irradiation (PubMed:17704809). Forms a tight complex with RANGAP1 and RANBP2 (PubMed:11853669, PubMed:15378033, PubMed:15608651, PubMed:15931224, PubMed:16732283). Identified in a complex with SUMO2 and UBE2I, where one ZNF451 interacts with one UBE2I and two SUMO2 chains, one bound to the UBE2I active site and the other to another region of the same UBE2I molecule (PubMed:12924945, PubMed:26524494). Interacts with SETX (PubMed:24105744). Interacts with HIPK1 and HIPK2 (By similarity). Interacts with PPM1J (By similarity). Interacts with RASD2 (By similarity). Interacts with TCF3 (By similarity). Interacts with NR2C1; the interaction promotes its sumoylation (By similarity). Interacts with SIAH1 (PubMed:9334332). Interacts with PARP (PubMed:9197546). Interacts with various transcription factors such as TFAP2A, TFAP2B, and TFAP2C (PubMed:12072434). Interacts with AR (PubMed:10383460). Interacts with ETS1 (PubMed:9333025). Interacts with SOX4 (PubMed:16631117). Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B (PubMed:17956732, PubMed:23469069). Interacts with FOXL2 (PubMed:19744555). Interacts with DNM1l (via its GTPase and B domains); the interaction promotes sumoylation of DNM1L, mainly in its B domain (PubMed:19638400). Interacts with NFATC2IP; this inhibits formation of poly-SUMO chains (PubMed:20077568). Interacts with FHIT (PubMed:11085938). Interacts with PRKRA and p53/TP53 (By similarity). Interacts with UHRF2 (PubMed:23404503). Interacts with NR3C1 and this interaction is enhanced in the presence of RWDD3 (PubMed:23508108, PubMed:25918163). Interacts with MTA1 (PubMed:21965678). Interacts with ZNF451 (PubMed:26524494). Interacts with CPEB3 (By similarity). Interacts with SUMO1, SUMO2 and SUMO3 (PubMed:17466333). Interacts with IPO13 (PubMed:21139563). Interacts with DNMT1 (PubMed:19450230). Interacts with SUMO1P1/SUMO5 (PubMed:27211601). Interacts with PML-RARA oncoprotein (via the coiled-colied domain); the interaction is required for sumoylation of the PML-RARA oncoprotein (PubMed:15809060). Interacts with ZBED1/hDREF (PubMed:27068747)

  • SwissProt ID

    P63279

  • Gene ID
    7329 [NCBI]
  • Synonyms

    UBE2I; UBC9; UBCE9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18

  • Research Field

    Cell Biology

SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane2(20μg), A549(20μg), A375(lane4(20μg) and HepG2(lane5(20μg) using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human rectum tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human duodenum tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling SUMO Conjugating Enzyme UBC9 with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling SUMO Conjugating Enzyme UBC9 with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image
    1/16
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane2(20μg), A549(20μg), A375(lane4(20μg) and HepG2(lane5(20μg) using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human rectum tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human small intestine tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human duodenum tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling SUMO Conjugating Enzyme UBC9 with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling SUMO Conjugating Enzyme UBC9 with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • Experimental Validation Results for SUMO Conjugating Enzyme UBC9 Antibody (YA3301)
  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane2(20μg), A549(20μg), A375(lane4(20μg) and HepG2(lane5(20μg) using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human rectum tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human stomach tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human duodenum tissue using SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, 1/200). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling SUMO Conjugating Enzyme UBC9 with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling SUMO Conjugating Enzyme UBC9 with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with SUMO Conjugating Enzyme UBC9 Antibody (HY-P83556) at 1/50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image WB
  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image IHC-P
  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image IHC-P
  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image IHC-P
  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image IHC-P
  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image IHC-P
  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image FC
  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image ICC
  • SUMO Conjugating Enzyme UBC9 Antibody (YA3301) Product Image ICC