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Cortactin Antibody (YA495)

(Synonyms: Amplaxin antibody; CTTN antibody; EMS 1 antibody; EMS1 antibody; FLJ34459 antibody; Mammary tumor and squamous cell carcinoma associated antibody; Oncogene EMS1 antibody; p80/85 src substrate antibody; Src substrate cortactin antibody; SRC8_HUMAN antibody)

Cortactin Antibody (YA495) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cortactin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, IP, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Cortactin Antibody (YA495) Product Image
ALL WB ICC IHC-P FC
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Cortactin Antibody (YA495) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:5000 1:100-1:500 1:50-1:200 1:50-1:100 Use at an assay dependent concentration.

Product Details

Beschreibung

Cortactin Antibody (YA495) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cortactin.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Cortactin.AA range:34-67.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102820

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Versand

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cortactin Antibody (YA495)
    Western blot analysis of extracts from Hela (lane 2(20μg) or (lane 3(40μg)) and NIH3T3 (lane 4(20μg) or (lane 5(40μg)) using Cortactin (HY-P80088) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Cortactin Antibody (YA495)
    Immunocytochemistry analysis of Hela cells labeling Cortactin with Cortactin antibody (HY-P80088) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cortactin antibody (HY-P80088) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cortactin Antibody (YA495)
    Immunocytochemistry analysis of Hela cells labeling Cortactin with Cortactin antibody (HY-P80088) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cortactin antibody (HY-P80088) at 1/250 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Cortactin Antibody (YA495)
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Cortactin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80088, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cortactin Antibody (YA495)
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Cortactin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80088, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cortactin Antibody (YA495)
    Flow cytometric analysis of 1X10^6 Hela cells labeling Cortactin Antibody (HY-P80088, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:21296879). Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (By similarity). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (PubMed:20861316). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity). Required for stabilization of KCNH1 channels at the cell membrane (PubMed:23144454). Plays a role in the invasiveness of cancer cells, and the formation of metastases (PubMed:16636290)

  • Subcellular Localization

    Cytoplasm, cytoskeleton; Cell projection, lamellipodium; Cell projection, ruffle; Cell projection, dendrite; Cell projection; Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, podosome; Cell junction; Cell junction, focal adhesion; Membrane, clathrin-coated pit; Cell projection, dendritic spine; Cytoplasm, cell cortex; Endoplasmic reticulum

  • Subunit

    Part of a complex composed of NEDD9, AURKA and CTTN; within the complex NEDD9 acts as a scaffold protein and is required for complex formation (PubMed:24574519). Interacts (via N-terminus) with NEDD9 (PubMed:24574519). Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Forms a complex made of ABL1 and MYLK (PubMed:20861316). Interacts with SHANK2 and SHANK3 (via its SH3 domain). Interacts with FGD1. Interacts with ABL2 (By similarity). Interacts with KCNA2 (via non-phosphorylated C-terminus) (PubMed:12151401). Interacts with PLXDC2 and SRCIN1. Interacts with SAMSN1 (via SH3 domain). Interacts (via SH3 domain) with ASAP1 (via Pro-rich region). Interacts (via SH3 domain) with DNM2 (By similarity). Interacts with ACTN1 (By similarity). Interacts with FER (PubMed:9722593). Interacts with CTTNBP2NL; this interaction may target CTTN to stress fibers. Interacts with CTTNBP2; this interaction may target CTTN at the cell cortex or dendritic spines. Interacts with KCNH1 (PubMed:23144454). Interacts (via SH3 domain) with DIP2A (via N-terminus); the interaction enhances CTTN acetylation and is required for proper synaptic transmission (By similarity). Interacts with XIRP1 (via N-terminus); the interaction promotes CTTN localization to intercalated disks in cardiomyocytes (By similarity)

  • SwissProt ID

    Q14247

  • Gene ID
    2017 [NCBI]
  • Synonyms

    Amplaxin antibody; CTTN antibody; EMS 1 antibody; EMS1 antibody; FLJ34459 antibody; Mammary tumor and squamous cell carcinoma associated antibody; Oncogene EMS1 antibody; p80/85 src substrate antibody; Src substrate cortactin antibody; SRC8_HUMAN antibody

  • Research Field

    Signal Transduction

Cortactin Antibody (YA495) Related Classifications

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    Cortactin Antibody (YA495) purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) or (lane 3(40μg)) and NIH3T3 (lane 4(20μg) or (lane 5(40μg)) using Cortactin (HY-P80088) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    Cortactin Antibody (YA495) purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Cortactin with Cortactin antibody (HY-P80088) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cortactin antibody (HY-P80088) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    Cortactin Antibody (YA495) purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Cortactin with Cortactin antibody (HY-P80088) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cortactin antibody (HY-P80088) at 1/250 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    Cortactin Antibody (YA495) purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Cortactin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80088, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    Cortactin Antibody (YA495) purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Cortactin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80088, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    Cortactin Antibody (YA495) purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling Cortactin Antibody (HY-P80088, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Cortactin Antibody (YA495) Product Image
    1/16
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Experimental Validation Results for Cortactin Antibody (YA495)
  • Cortactin Antibody (YA495) Product Image
  • Cortactin Antibody (YA495) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) or (lane 3(40μg)) and NIH3T3 (lane 4(20μg) or (lane 5(40μg)) using Cortactin (HY-P80088) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Cortactin Antibody (YA495) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Cortactin with Cortactin antibody (HY-P80088) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cortactin antibody (HY-P80088) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cortactin Antibody (YA495) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Cortactin with Cortactin antibody (HY-P80088) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cortactin antibody (HY-P80088) at 1/250 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Cortactin Antibody (YA495) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Cortactin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80088, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cortactin Antibody (YA495) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using Cortactin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80088, 1/200) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cortactin Antibody (YA495) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling Cortactin Antibody (HY-P80088, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Cortactin Antibody (YA495) Product Image WB
  • Cortactin Antibody (YA495) Product Image ICC
  • Cortactin Antibody (YA495) Product Image ICC
  • Cortactin Antibody (YA495) Product Image IHC-P
  • Cortactin Antibody (YA495) Product Image IHC-P
  • Cortactin Antibody (YA495) Product Image FC