beta II Tubulin Antibody (YA6843)

Cat. No.: HY-P87150: Data Sheet User Guide for Antibodies
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beta II Tubulin Antibody (YA6843) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to beta II Tubulin.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

beta II Tubulin Antibody (YA6843) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to beta II Tubulin.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 50kDa;

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q13509

Gene ID

10383 [NCBI]

Immunogen

A synthesized peptide derived from human beta III Tubulin. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-10000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-500
FC FC: Flow Cytometry	1:50-200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers (PubMed:34996871, PubMed:38305685, PubMed:38609661). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871, PubMed:38305685, PubMed:38609661). Below the cap, alpha-beta tubulin heterodimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871, PubMed:38609661). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977)

Subcellular Localization：Cytoplasm, cytoskeleton,Cell projection, growth cone,Cell projection, lamellipodium,Cell projection, filopodium

Expression：
Tissue_Specificity: Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues

Isoforms & Post-Translational Modification：Q13509 has two isomers: Q13509-1: 50433 Da (predicted); Q13509-2: 42433 Da (predicted).
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866)丨Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human丨Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase

Subunit：Heterodimer of alpha- and beta-tubulin (PubMed:34996871, PubMed:35482892, PubMed:38305685, PubMed:38609661)

Synonyms

bA506K6.1 antibody; Beta2 antibody; class II beta tubulin isotype antibody; class IIa beta-tubulin antibody; class IIb beta-tubulin antibody; Class IVb beta tubulin antibody; dJ40E16.7 antibody; DKFZp566F223 antibody; FLJ98847 antibody; M(beta)2 antibody; bA506K6.1 antibody; Beta2 antibody; class II beta tubulin isotype antibody; class IIa beta-tubulin antibody; class IIb beta-tubulin antibody; Class IVb beta tubulin antibody; dJ40E16.7 antibody; DKFZp566F223 antibody; FLJ98847 antibody; M(beta)2 antibody; MGC8685 antibody; OTTHUMP00000015956 antibody; OTTHUMP00000015964 antibody; TBB4B_HUMAN antibody; TUBB 2 antibody; TUBB 2A antibody; TUBB 2C antibody; TUBB antibody; TUBB PARALOG antibody; TUBB2 antibody; TUBB2A antibody; TUBB2B antibody; TUBB2C antibody; Tubb4b antibody; Tubulin beta 2 antibody; Tubulin beta 2 chain antibody; Tubulin beta 2A antibody; Tubulin beta 2A chain antibody; Tubulin beta 2B antibody; Tubulin beta 2B chain antibody; Tubulin beta 2C antibody; Tubulin beta polypeptide antibody; Tubulin beta polypeptide 2 antibody; Tubulin beta polypeptide paralog antibody; Tubulin beta-2 chain antibody; Tubulin beta-2C chain antibody; Tubulin beta-4B chain antibody; tubulin, beta 2A class IIa antibody; tubulin, beta 2B class IIb antibody; tubulin, beta 4B class IVb antibody; Tubulin, beta, class IVB antibody; Tubulin, beta-4B antibody; beta Ⅱ Tubulin beta Ⅱ Tubulin

Documentation

User Guide for Antibodies (1077 KB)