Aquaporin 4 Antibody (YA5349)

Cat. No.: HY-P85657: Data Sheet COA
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Aquaporin 4 Antibody (YA5349) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Aquaporin 4.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Aquaporin 4 Antibody (YA5349) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Aquaporin 4.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 48 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P55087

Gene ID

361 [NCBI]

Immunogen

Synthetic Peptide of Aquaporin 4

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-200
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-300

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Forms a water-specific channel (PubMed:19383790, PubMed:7559426, PubMed:8601457). Plays an important role in brain water homeostasis (PubMed:37143309). It is involved in glymphatic solute transport and is required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability (By similarity)

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein; Endosome membrane; Cell membrane, sarcolemma; Multi-pass membrane protein; Cell projection

Expression：
Tissue_specificity:Detected in skeletal muscle (PubMed:29055082) . Detected in the stomach, along the basal region of the fundic glands (protein level) (PubMed:8601457) . Detected in the brain, lungs, and skeletal muscle, with extremely low levels in the heart and ovaries (PubMed:7559426, PubMed:8601457) .

Isoforms & Post-Translational Modification：P55087 has 2 isomers: P55087-1: 34830 Da (predicted); P55087-2: 32300 Da (predicted).
Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamate increases conductance by 40% (By similarity);Isoform 2: Palmitoylated on its N-terminal region. Isoform 1: Not palmitoylated

Subunit：Homotetramer (PubMed:19383790). The tetramers can form oligomeric arrays in membranes. The size of the oligomers differs between tissues and is smaller in skeletal muscle than in brain. Interaction between AQP4 oligomeric arrays in close-by cells can contribute to cell-cell adhesion (By similarity). Part of a complex containing MLC1, TRPV4, HEPACAM and ATP1B1 (PubMed:22328087)

Synonyms

AQP4; Aquaporin-4; AQP-4; Mercurial-insensitive water channel; MIWC; WCH4

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)