USP25 Antibody
(Synonyms: USP21, USP25, Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP on chromosome 21, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25)USP25 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to USP25.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
USP25 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to USP25.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 130 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 122; 125; 129 kDa
Purified recombinant protein of human USP25
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
USP25 is a Deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates and thus, functions in various biological processes including inflammation and immune response. Modulates the Wnt/beta-catenin pathway by deubiquitinating and stabilizing tankyrases TNKS1 and TNKS2. Regulates KEAP1-NRF2 axis in the defense against oxidative assaults by deubiquitinating KEAP1 and protecting it from degradation leading to degradation of the NRF2 transcription factor that is responsible for mounting an anti-oxidation gene expression program. Positively regulates RNA virus-induced innate signaling by interacting with and deubiquitinating ERLIN1 and ERLIN2. In turn, restricts virus production by regulating cholesterol biosynthetic flux. Acts as a negative regulator of interleukin-17-mediated signaling and inflammation through the removal of 'Lys-63'-linked ubiquitination of TRAF5 and TRAF6. Prevents the ubiquitination and degradation of TRAF3 to reduce the phosphorylation levels of JNK and P38, the secretion of IL-1B and to induce endotoxin tolerance[1][2][3][4][5][6][7][8].
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Subcellular Localization
Cytoplasm
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Expression
Tissue_Specificity: Isoform USP25a is found in most adult and fetal tissues; expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Expressed in the brain, with high levels in the cerebral cortex. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.
Induction: The muscle-specific isoform (USP25m) is up-regulated during myocyte differentiation. Levels increase up to 100-fold towards completion of differentiation. -
Isoforms & Post-Translational Modification
USP25 has 3 isoforms, Q9UHP3-2: amino acid length is 1055, molecular weight is 122218 Da (predicted); Q9UHP3-1: amino acid length is 1087, molecular weight is 125750 Da (predicted); Q9UHP3-3: amino acid length is 1125, molecular weight is 129963 Da (predicted).发生乙酰化修饰USP25 存在 3 个异构体，Q9UHP3-2：氨基酸个数为 1055 个，分子量为 122218 Da (预测)；Q9UHP3-1：氨基酸个数为 1087 个，分子量为 125750 Da (预测)；Q9UHP3-3：氨基酸个数为 1125 个，分子量为 129963 Da (预测)。Acetylated
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Subunit
Homotetramer, inhibited form.
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SwissProt ID
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Synonyms
USP21, USP25, Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP on chromosome 21, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25
Documentation
[1]. Qian G, et al. Smurf1 restricts the antiviral function mediated by USP25 through promoting its ubiquitination and degradation. Biochem Biophys Res Commun. 2018 Apr 6;498(3):537-543. [Content Brief]
[2]. Teo QW, et al. Usp25-Erlin1/2 activity limits cholesterol flux to restrict virus infection. Dev Cell. 2023 Nov 20;58(22):2495-2509.e6. [Content Brief]
[3]. Xu D, et al. USP25 regulates Wnt signaling by controlling the stability of tankyrases. Genes Dev. 2017 May 15;31(10):1024-1035. [Content Brief]
[4]. Sauer F, et al. Differential Oligomerization of the Deubiquitinases USP25 and USP28 Regulates Their Activities. Mol Cell. 2019 May 2;74(3):421-435.e10. [Content Brief]
[5]. Fan CX, et al. Heterozygous variants in USP25 cause genetic generalized epilepsy. Brain. 2024 Oct 3;147(10):3442-3457. [Content Brief]
[6]. Cai C, et al. USP25 regulates KEAP1-NRF2 anti-oxidation axis and its inactivation protects acetaminophen-induced liver injury in male mice. Nat Commun. 2023 Jun 20;14(1):3648. [Content Brief]
[7]. Zhong B, et al. Negative regulation of IL-17-mediated signaling and inflammation by the ubiquitin-specific protease USP25. Nat Immunol. 2012 Nov;13(11):1110-7. [Content Brief]
[8]. Wen J, et al. USP25 promotes endotoxin tolerance via suppressing K48-linked ubiquitination and degradation of TRAF3 in Kupffer cells. Mol Immunol. 2019 Feb;106:53-62. [Content Brief]
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