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GPR30 Antibody

(Synonyms: G Protein Coupled Receptor 30; G-protein coupled receptor 30; G-protein coupled estrogen receptor 1; Membrane estrogen receptor; mER; Chemokine receptor-like 2; IL8-related receptor DRY12; Flow-induced endothelial G-protein coupled receptor 1; FEG-1; Lymphocyte-derived G-protein coupled receptor; LYGPR; GPCR-BR; CEPR; CMKRL2; DRY12; GPER.)
Customer Review

Based on 1 Customer Validation

GPR30 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to GPR30.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
ALL FC ICC
  • Experimental Validation Results for GPR30 Antibody
  • Experimental Validation Results for GPR30 Antibody
  • Experimental Validation Results for GPR30 Antibody
  • GPR30 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
FC Info
FC: Flow Cytometry
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:5000-10000 1:100-500 1:100-500 1µg:Test 1:100 1:100-500

Product Details

Description

GPR30 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to GPR30.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat Predicted Reactivity: Chicken,Cow,Horse,Rabbit
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthetic peptide derived from human GPR30: 251-375/375

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103075

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GPR30 Antibody
    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling GPR30 Antibody (HY-P81150, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/Test dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for GPR30 Antibody
    Immunocytochemistry analysis of SH-SY5Y cells labeling GPR30 with GPR30 Antibody (HY-P81150) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GPR30 Antibody (HY-P81150) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GPR30 Antibody
    Immunocytochemistry analysis of SH-SY5Y cells labeling GPR30 with GPR30 Antibody (HY-P81150) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GPR30 Antibody (HY-P81150) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    GPR30 is a G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK. Mediates pleiotropic functions among others in the cardiovascular, endocrine, reproductive, immune and central nervous systems. Has a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a RAMP3-dependent manner. Regulates arterial blood pressure by stimulating vasodilation and reducing vascular smooth muscle and microvascular endothelial cell proliferation. Plays a role in blood glucose homeostasis contributing to the insulin secretion response by pancreatic beta cells. Triggers mitochondrial apoptosis during pachytene spermatocyte differentiation. Stimulates uterine epithelial cell proliferation. Enhances uterine contractility in response to oxytocin. Contributes to thymic atrophy by inducing apoptosis. Attenuates TNF-mediated endothelial expression of leukocyte adhesion molecules. Promotes neuritogenesis in developing hippocampal neurons. Plays a role in acute neuroprotection against NMDA-induced excitotoxic neuronal death. Increases firing activity and intracellular calcium oscillations in luteinizing hormone-releasing hormone (LHRH) neurons. Inhibits early osteoblast proliferation at growth plate during skeletal development. Inhibits mature adipocyte differentiation and lipid accumulation. Involved in the recruitment of beta-arrestin 2 ARRB2 at the plasma membrane in epithelial cells. Also functions as a receptor for aldosterone mediating rapid regulation of vascular contractibility through the PI3K/ERK signaling pathway. Involved in cancer progression regulation. Stimulates cancer-associated fibroblast (CAF) proliferation by a rapid genomic response through the EGFR/ERK transduction pathway. Associated with EGFR, may act as a transcription factor activating growth regulatory genes (c-fos, cyclin D1). Promotes integrin alpha-5/beta-1 and fibronectin (FN) matrix assembly in breast cancer cells

  • Subcellular Localization

    Nucleus; Cytoplasm; Cytoplasm, perinuclear region; Cytoplasm, cytoskeleton; Cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein; Cytoplasmic vesicle membrane; Multi-pass membrane protein; Early endosome; Recycling endosome; Golgi apparatus membrane; Multi-pass membrane protein; Golgi apparatus, trans-Golgi network; Endoplasmic reticulum membrane; Multi-pass membrane protein; Cell projection, dendrite; Cell projection, dendritic spine membrane; Multi-pass membrane protein; Cell projection, axon; Postsynaptic density; Mitochondrion membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:This gene is expressed (protein level) in the placenta, endothelial and epithelial cells, myometrium during non-labor and labor, fibroblasts and cancer-associated fibroblasts (CAF) , prostate cancer cells, and invasive adenocarcinoma cells. It is universally expressed, but most abundantly in the placenta. In brain regions, it is expressed as a 2.8 kb transcript in the basal forebrain, frontal cortex, thalamus, hippocampus, caudate nucleus, and putamen.

    Induction:Up-regulated by EGF and TGF-alpha in endometrial, ovarian and breast tumor cells. Up-regulated by progestin and by phorbol 12-myristate 13-acetate (PMA) in breast cancer cell lines

  • Subunit

    Homodimer (Probable). Heterodimer; heterodimerizes with other G-protein-coupled receptor (GPCRs) like CRHR1, HTR1A and PAQR8. Interacts (via C-terminus tail motif) with DLG4 (via N-terminus tandem pair of PDZ domains); the interaction is direct and induces the increase of GPER1 protein levels residing at the plasma membrane surface in a estradiol-independent manner (By similarity). Interacts with RAMP3; the interaction confers proper subcellular localization and function in cardioprotection. Interacts with KRT7 and KRT8. Interacts with EGFR; the interaction increases after agonist-induced stimulation in cancer-associated fibroblasts (CAF). Interacts with EGFR and ESR1

  • SwissProt ID

    Q99527

  • Gene ID
    2852 [NCBI]
  • Synonyms

    G Protein Coupled Receptor 30; G-protein coupled receptor 30; G-protein coupled estrogen receptor 1; Membrane estrogen receptor; mER; Chemokine receptor-like 2; IL8-related receptor DRY12; Flow-induced endothelial G-protein coupled receptor 1; FEG-1; Lymphocyte-derived G-protein coupled receptor; LYGPR; GPCR-BR; CEPR; CMKRL2; DRY12; GPER.

GPR30 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling GPR30 Antibody (HY-P81150, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/Test dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling GPR30 with GPR30 Antibody (HY-P81150) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GPR30 Antibody (HY-P81150) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling GPR30 with GPR30 Antibody (HY-P81150) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GPR30 Antibody (HY-P81150) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • GPR30 Antibody Product Image
    1/16
  • Experimental Validation Results for GPR30 Antibody
  • Experimental Validation Results for GPR30 Antibody
  • Experimental Validation Results for GPR30 Antibody
  • GPR30 Antibody Product Image
  • Experimental Validation Results for GPR30 Antibody
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 SH-SY5Y cells labeling GPR30 Antibody (HY-P81150, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/Test dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for GPR30 Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling GPR30 with GPR30 Antibody (HY-P81150) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GPR30 Antibody (HY-P81150) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GPR30 Antibody
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling GPR30 with GPR30 Antibody (HY-P81150) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GPR30 Antibody (HY-P81150) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GPR30 Antibody
  • Experimental Validation Results for GPR30 Antibody
  • Experimental Validation Results for GPR30 Antibody
  • GPR30 Antibody Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 SH-SY5Y cells labeling GPR30 Antibody (HY-P81150, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/Test dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • GPR30 Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of SH-SY5Y cells labeling GPR30 with GPR30 Antibody (HY-P81150) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GPR30 Antibody (HY-P81150) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • GPR30 Antibody Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of SH-SY5Y cells labeling GPR30 with GPR30 Antibody (HY-P81150) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with GPR30 Antibody (HY-P81150) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • GPR30 Antibody Product Image FC
  • GPR30 Antibody Product Image ICC
  • GPR30 Antibody Product Image ICC