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Aromatase Antibody (YA611)

(Synonyms: CYP19A1; ARO1; CYAR; CYP19; Cytochrome P450 19A1; Aromatase; CYPXIX; Cytochrome P-450AROM; Estrogen synthase)
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Based on 1 publication(s) in Google Scholar

Aromatase Antibody (YA611) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Aromatase.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Aromatase Antibody (YA611)
  • Aromatase Antibody (YA611) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) Aromatase Antibody (YA611)

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

Aromatase Antibody (YA611) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Aromatase.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 58 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Aromatase.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102603

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Aromatase Antibody (YA611)
    Western blot analysis of extracts from MCF-7(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Aromatase Antibody (HY-P80547) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Aromatase is an A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase)[1][2][3][4].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein; Microsome membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Widely expressed, including in human brain, placenta, skin fibroblasts, adipose tissue and gonads

  • Isoforms & Post-Translational Modification

    P11511 has 2 isomers: P11511-1: 57883 Da (predicted); P11511-2: 24518 Da (predicted).
    Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1)

  • SwissProt ID

    P11511

  • Gene ID
    1588 [NCBI]
  • Synonyms

    CYP19A1; ARO1; CYAR; CYP19; Cytochrome P450 19A1; Aromatase; CYPXIX; Cytochrome P-450AROM; Estrogen synthase

  • Research Field

    Cell Biology

[1]. Baravalle R, et al. Impact of R264C and R264H polymorphisms in human aromatase function. J Steroid Biochem Mol Biol. 2017 Mar;167:23-32. [Content Brief]

[2]. Corbin CJ, et al. Isolation of a full-length cDNA insert encoding human aromatase system cytochrome P-450 and its expression in nonsteroidogenic cells. Proc Natl Acad Sci U S A. 1988 Dec;85(23):8948-52. [Content Brief]

[3]. Sohl CD, et al. Kinetic analysis of the three-step steroid aromatase reaction of human cytochrome P450 19A1. J Biol Chem. 2010 Jun 4;285(23):17734-43. [Content Brief]

[4]. Cheng Q, et al. Oxidation of dihydrotestosterone by human cytochromes P450 19A1 and 3A4. J Biol Chem. 2012 Aug 24;287(35):29554-67. [Content Brief]

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Aromatase Antibody (YA611) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Aromatase Antibody (HY-P80547) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Aromatase Antibody (YA611) Product Image
    1/16
  • Experimental Validation Results for Aromatase Antibody (YA611)
  • Aromatase Antibody (YA611) Product Image
  • Experimental Validation Results for Aromatase Antibody (YA611)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Aromatase Antibody (HY-P80547) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Aromatase Antibody (YA611)
  • Aromatase Antibody (YA611) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Aromatase Antibody (HY-P80547) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Aromatase Antibody (YA611) Product Image WB