2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Aromatase Antibody (YA611)

Aromatase Antibody (YA611) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Aromatase.

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Top Publications Citing Use of Products

1 Publications Citing Use of MCE Aromatase Antibody (YA611)

  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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  • Descripciòn

Descripciòn

Aromatase Antibody (YA611) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Aromatase.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Peso molecular
Predicted band size: 58 kDa;
Observed band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Synthetic peptide corresponding to Human Aromatase.The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Pureza affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from MCF-7(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Aromatase Antibody (HY-P80547) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively (PubMed:27702664, PubMed:2848247). Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid (PubMed:20385561). Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen (PubMed:22773874). Also displays 2-hydroxylase activity toward estrone (PubMed:22773874). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:20385561, PubMed:22773874)
Subcellular Localization：Endoplasmic reticulum membrane; Multi-pass membrane protein; Microsome membrane; Multi-pass membrane protein
Expression：
Tissue_specificity:Widely expressed, including in human brain, placenta, skin fibroblasts, adipose tissue and gonads
Isoforms & Post-Translational Modification：P11511 has 2 isomers: P11511-1: 57883 Da (predicted); P11511-2: 24518 Da (predicted).
Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1)
RRID
Database

Entrez Gene: 1588 Human ; 13075 Mouse ;

SwissProt: P11511 Human ; P28649 Mouse ; P22443 Rat

OMIM: 139300 Human

Research Field

Cell Biology
Synonyms
CYP19A1; ARO1; CYAR; CYP19; Cytochrome P450 19A1; Aromatase; CYPXIX; Cytochrome P-450AROM; Estrogen synthase
Documentación
SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Aromatase Antibody (YA611) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

Nombre del producto:
Aromatase Antibody (YA611)
Cat. No.:
HY-P80547
Cantidad:
MCE Japan Authorized Agent:

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