Aromatase Antibody (YA611)

Cat. No.: HY-P80547: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Aromatase Antibody (YA611) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Aromatase.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Aromatase Antibody (YA611) Featured Recommendations:

1 Publications Citing Use of MCE Aromatase Antibody (YA611)

•Theriogenology. 2026 Aug:260:117913.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Aromatase Antibody (YA611) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Aromatase.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 58 kDa;

Observed band size: 50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P11511

Gene ID

1588 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Aromatase.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from MCF-7(lane 2(20ug) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Aromatase Antibody (HY-P80547) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively (PubMed:27702664, PubMed:2848247). Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid (PubMed:20385561). Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen (PubMed:22773874). Also displays 2-hydroxylase activity toward estrone (PubMed:22773874). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:20385561, PubMed:22773874)

Subcellular Localization：Endoplasmic reticulum membrane; Multi-pass membrane protein; Microsome membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Widely expressed, including in human brain, placenta, skin fibroblasts, adipose tissue and gonads

Isoforms & Post-Translational Modification：P11511 has 2 isomers: P11511-1: 57883 Da (predicted); P11511-2: 24518 Da (predicted).
Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1)

RRID

AB_3102603

Database

Entrez Gene: 1588 Human ; 13075 Mouse ;

SwissProt: P11511 Human ; P28649 Mouse ; P22443 Rat

OMIM: 139300 Human

Research Field

Cell Biology

Synonyms

CYP19A1; ARO1; CYAR; CYP19; Cytochrome P450 19A1; Aromatase; CYPXIX; Cytochrome P-450AROM; Estrogen synthase

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Aromatase Antibody (YA611) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Aromatase Antibody (YA611)

Aromatase Antibody (YA611) Featured Recommendations:

1 Publications Citing Use of MCE Aromatase Antibody (YA611)

Product Detail

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Background

Documentation

Aromatase Antibody (YA611) Related Classifications

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