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TIMP3 Antibody (YA6800)

(Synonyms: HSMRK222 antibody; K222 antibody; K222TA2 antibody; Metalloproteinase inhibitor 3 antibody; MIG 5 protein antibody; MIG5 protein antibody; Protein MIG 5 antibody; Protein MIG-5 antibody; SFD antibody; Sorsby fundus dystrophy pseudoinflammatory antibody; HSMRK222 antibody; K222 antibody; K222TA2 antibody; Metalloproteinase inhibitor 3 antibody; MIG 5 protein antibody; MIG5 protein antibody; Protein MIG 5 antibody; Protein MIG-5 antibody; SFD antibody; Sorsby fundus dystrophy pseudoinflammatory antibody; TIMP 3 antibody; TIMP metallopeptidase inhibitor 3 antibody; TIMP-3 antibody; TIMP3 antibody; TIMP3_HUMAN antibody; Tissue Inhibitor of Metalloproteinase 3 antibody; Tissue inhibitor of metalloproteinases 3 antibody; Tissue inhibitor of metalloproteinases3 antibody; )
Customer Review

Based on 1 Customer Validation

TIMP3 Antibody (YA6800) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIMP3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for TIMP3 Antibody (YA6800)
  • TIMP3 Antibody (YA6800) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000 1:100

Product Details

Description

TIMP3 Antibody (YA6800) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIMP3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 20 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 24 kDa
Immunogen

Recombinant protein within human TIMP3 aa 1-211 / 211.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719262

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TIMP3 Antibody (YA6800)
    Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、A431 (lane 2(20μg)) 、Rat brain (lane 3(20μg)) and Rat spleen (lane 4(20μg)) using TIMP3 Antibody (HY-P87107) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    TIMP3 mediates a variety of processes including matrix regulation and turnover, inflammation, and angiogenesis, through reversible inhibition of zinc protease superfamily enzymes, primarily matrix metalloproteinases (MMPs). Regulates extracellular matrix (ECM) remodeling through inhibition of matrix metalloproteinases (MMP) including MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. Additionally, modulates the processing of amyloid precursor protein (APP) and apolipoprotein E receptor ApoER2 by inhibiting two alpha-secretases ADAM10 and ADAM17. Functions as a tumor suppressor and a potent inhibitor of angiogenesis. Exerts its anti-angiogenic effect by directly interacting with vascular endothelial growth factor (VEGF) receptor-2/KDR, preventing its binding to the VEGFA ligand. Selectively induces apoptosis in angiogenic endothelial cells through a caspase-independent cell death pathway. Mechanistically, inhibits matrix-induced focal adhesion kinase PTK2 tyrosine phosphorylation and association with paxillin/PXN and disrupts the incorporation of ITGB3, PTK2 and PXN into focal adhesion contacts on the matrix[1][2][3].

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • Isoforms & Post-Translational Modification

    P35625: 211 amino acids, molecular weight 24145 Da.

  • Subunit

    Interacts with EFEMP1

  • SwissProt ID

    P35625

  • Gene ID
    7078 [NCBI]
  • Synonyms

    HSMRK222 antibody; K222 antibody; K222TA2 antibody; Metalloproteinase inhibitor 3 antibody; MIG 5 protein antibody; MIG5 protein antibody; Protein MIG 5 antibody; Protein MIG-5 antibody; SFD antibody; Sorsby fundus dystrophy pseudoinflammatory antibody; HSMRK222 antibody; K222 antibody; K222TA2 antibody; Metalloproteinase inhibitor 3 antibody; MIG 5 protein antibody; MIG5 protein antibody; Protein MIG 5 antibody; Protein MIG-5 antibody; SFD antibody; Sorsby fundus dystrophy pseudoinflammatory antibody; TIMP 3 antibody; TIMP metallopeptidase inhibitor 3 antibody; TIMP-3 antibody; TIMP3 antibody; TIMP3_HUMAN antibody; Tissue Inhibitor of Metalloproteinase 3 antibody; Tissue inhibitor of metalloproteinases 3 antibody; Tissue inhibitor of metalloproteinases3 antibody;

TIMP3 Antibody (YA6800) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、A431 (lane 2(20μg)) 、Rat brain (lane 3(20μg)) and Rat spleen (lane 4(20μg)) using TIMP3 Antibody (HY-P87107) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • TIMP3 Antibody (YA6800) Product Image
    1/16
  • Experimental Validation Results for TIMP3 Antibody (YA6800)
  • TIMP3 Antibody (YA6800) Product Image
  • Experimental Validation Results for TIMP3 Antibody (YA6800)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、A431 (lane 2(20μg)) 、Rat brain (lane 3(20μg)) and Rat spleen (lane 4(20μg)) using TIMP3 Antibody (HY-P87107) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TIMP3 Antibody (YA6800)
  • TIMP3 Antibody (YA6800) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HepG2 (lane 1(20μg)) 、A431 (lane 2(20μg)) 、Rat brain (lane 3(20μg)) and Rat spleen (lane 4(20μg)) using TIMP3 Antibody (HY-P87107) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • TIMP3 Antibody (YA6800) Product Image WB