Cytokeratin 8 Antibody (YA3005)

Cat. No.: HY-P83260: Data Sheet COA
SDS
User Guide for Antibodies
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Cytokeratin 8 Antibody (YA3005) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 8.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Cytokeratin 8 Antibody (YA3005) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Cytokeratin 8 Antibody (YA3005) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cytokeratin 8.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 54 kDa;

Observed band size: 54 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P05787

Gene ID

3856 [NCBI]

Immunogen

A synthesized peptide derived from human Cytokeratin 8 aa450-483.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:50
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), A549 (lane 2, 15 μg), and MCF-7 (lane 3, 15 μg) using Cytokeratin 8 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Western blot analysis of extracts from Hela(lane 2(20ug) and Jurkat(lane 3(20ug) using Cytokeratin 8 Antibody (HY-P83260) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling Cytokeratin 8 with Cytokeratin 8 Antibody (HY-P83260) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 8 Antibody (HY-P83260) at 1/50 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of MCF-7 cells labeling Cytokeratin 8 with Cytokeratin 8 Antibody (HY-P83260) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cytokeratin 8 Antibody (HY-P83260) at 1/50 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle

Subcellular Localization：Cytoplasm; Nucleus, nucleoplasm; Nucleus matrix

Expression：
Tissue_specificity:Aggregates of dystrophin and actin are observed in muscle fibers at the sarcoplasmic sarcomere and sarcomere. They are also expressed in the gingival mucosa and hard palate of the oral cavity.

Isoforms & Post-Translational Modification：P05787 has 2 isomers: P05787-1: 53704 Da (predicted); P05787-2: 56608 Da (predicted).
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization;O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation;O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner

Subunit：Heterotetramer of two type I and two type II keratins (PubMed:16000376). Forms a heterodimer with KRT18 (PubMed:10852826, PubMed:24940650). Associates with KRT20 (PubMed:10973561, PubMed:16608857). Interacts with PLEC isoform 1C, when in a heterodimer with KRT18 (PubMed:24940650). Interacts with PNN (PubMed:10809736). When associated with KRT19, interacts with DMD. Interacts with TCHP (PubMed:15731013). Interacts with APEX1 (PubMed:19188445). Interacts with GPER1 (PubMed:21149639). Interacts with EPPK1 (By similarity). Interacts with PKP1 and PKP2 (PubMed:10852826)

RRID

AB_3105374

Database

Entrez Gene: 3856 Human ; 16691 Mouse ; 25626 Rat

SwissProt: P05787 Human ; P11679 Mouse ; Q10758 Rat

OMIM: 215600 Human

Research Field

Signal Transduction

Synonyms

KRT8; CYK8; Keratin; type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Cytokeratin 8 Antibody (YA3005) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cytokeratin 8 Antibody (YA3005)

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Cytokeratin 8 Antibody (YA3005) Related Classifications

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