Toll-Like Receptor 3 Antibody (YA5327)

Cat. No.: HY-P85635: Data Sheet User Guide for Antibodies
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Toll-Like Receptor 3 Antibody (YA5327) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Toll-Like Receptor 3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Toll-Like Receptor 3 Antibody (YA5327) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Toll-Like Receptor 3.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 104 kDa;

Species Reactivity

Transfected

SwissProt ID

O15455

Gene ID

7098 [NCBI]

Immunogen

Purified recombinant human Toll-Like Receptor 3 protein fragments expressed in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Sensitivity	Transfected	Purity	affinity chromatography.
Conjugation	Non-conjugated	Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response

Subcellular Localization：Endoplasmic reticulum membrane; Single-pass type I membrane protein; Endosome membrane; Early endosome

Expression：
Tissue_specificity:It is highly expressed in the placenta and pancreas. It can also be detected in CD11c+ immature dendritic cells. It is expressed only in dendritic cells and not in other leukocytes (including monocyte precursors) . TLR3 is the most strongly expressed TLR in the brain, especially in astrocytes, glial cells, and neurons.

Isoforms & Post-Translational Modification：O15455 has 2 isomers: O15455-1: 103829 Da (predicted); O15455-2: 72937 Da (predicted).
Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding;TLR3 signaling requires a proteolytic cleavage mediated by cathepsins CTSB and CTSH, the cleavage occurs between amino acids 252 and 346. The cleaved form of TLR3 is the predominant form found in endosomes;Ubiquitinated by TRIM3; leading to recognition and sorting of polyubiquitinated TLR3 by the ESCRT complexes (PubMed:32878999). Ubiquitinated by ZNRF1 via 'Lys-63'-linked ubiquitin chains; leading to TLR3 lysosomal trafficking and degradation (PubMed:37158982). Ubiquitinated by RNF170 at Lys-765 via 'Lys-48'-linked ubiquitin chains; leading to TLR3 proteasomal degradation (PubMed:31076723)

Subunit：Monomer and homodimer; dimerization is triggered by ligand-binding, the signaling unit is composed of one ds-RNA of around 40 bp and two TLR3 molecules, and lateral clustering of signaling units along the length of the ds-RNA ligand is required for TLR3 signal transduction. Interacts (via transmembrane domain) with UNC93B1; the interaction is required for transport from the ER to the endosomes (PubMed:33432245). Interacts with SRC; upon binding of double-stranded RNA. Interacts with TICAM1 (via the TIR domain) in response to poly(I:C) and this interaction is enhanced in the presence of WDFY1 (PubMed:25736436). The tyrosine-phosphorylated form (via TIR domain) interacts with WDFY1 (via WD repeat 2) in response to poly(I:C) (PubMed:25736436)

Synonyms

CD283; CD283 antigen; IIAE2; TLR 3; Tlr3; TLR3_HUMAN; Toll Like Receptor 3; Toll-like receptor 3.

Documentation

User Guide for Antibodies (1077 KB)