GNE Antibody (YA2917)

Cat. No.: HY-P83172: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

GNE Antibody (YA2917) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GNE.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

GNE Antibody (YA2917) Featured Recommendations:

1 Publications Citing Use of MCE GNE Antibody (YA2917)

•MAbs. 2025 Dec;17(1):2561823. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

GNE Antibody (YA2917) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GNE.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 79 kDa;

Observed band size: 75 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9Y223

Gene ID

10020 [NCBI]

Immunogen

A synthetic peptide of human GLCNE

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg) using GNE (HY-P83172) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Bifunctional enzyme that possesses both UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities, and serves as the initiator of the biosynthetic pathway leading to the production of N-acetylneuraminic acid (NeuAc), a critical precursor in the synthesis of sialic acids. By catalyzing this pivotal and rate-limiting step in sialic acid biosynthesis, this enzyme assumes a pivotal role in governing the regulation of cell surface sialylation, playing a role in embryonic angiogenesis (PubMed:10334995, PubMed:11326336, PubMed:14707127, PubMed:16503651, PubMed:2808337, PubMed:38237079). Sialic acids represent a category of negatively charged sugars that reside on the surface of cells as terminal components of glycoconjugates and mediate important functions in various cellular processes, including cell adhesion, signal transduction, and cellular recognition (PubMed:10334995, PubMed:14707127)

Subcellular Localization：Cytoplasm, cytosol

Expression：
Tissue_specificity:Highest expression in liver and placenta. Also found in heart, brain, lung, kidney, skeletal muscle and pancreas. Isoform 1 is expressed in heart, brain, kidney, liver, placenta, lung, spleen, pancreas, skeletal muscle and colon. Isoform 2 is expressed mainly in placenta, but also in brain, kidney, liver, lung, pancreas and colon. Isoform 3 is expressed at low level in kidney, liver, placenta and colon

Isoforms & Post-Translational Modification：Q9Y223 has 5 isomers: Q9Y223-1: 79275 Da (predicted); Q9Y223-2: 83066 Da (predicted); Q9Y223-3: 78579 Da (predicted); Q9Y223-4: 71278 Da (predicted); Q9Y223-5: 66784 Da (predicted).
Phosphorylated. Phosphorylation by PKC activates the UDP-N-acetylglucosamine 2-epimerase activity

Subunit：Homodimer (PubMed:19841673, PubMed:22343627). Homotetramer (PubMed:26980148). Homohexamer (PubMed:19841673). The hexameric form exhibits both enzyme activities, whereas the dimeric form only catalyzes the phosphorylation of N-acyl-D-mannosamine (By similarity)

RRID

AB_3105287

Database

Entrez Gene: 10020 Human

SwissProt: Q9Y223 Human

OMIM: 269921 Human

Research Field

Cardiovascular

Synonyms

NM; DMRV; IBM2; Uae1; GLCNE

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

GNE Antibody (YA2917) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Country or Region *

Email Address *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

GNE Antibody (YA2917)

GNE Antibody (YA2917) Featured Recommendations:

1 Publications Citing Use of MCE GNE Antibody (YA2917)

Product Detail

Verification Image

Background

Documentation

GNE Antibody (YA2917) Related Classifications

Your Recently Viewed Products:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report