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GNE Antibody (YA2917)

(Synonyms: NM; DMRV; IBM2; Uae1; GLCNE)
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Based on 1 publication(s) in Google Scholar

GNE Antibody (YA2917) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GNE.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for GNE Antibody (YA2917)
  • GNE Antibody (YA2917) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:20

Product Details

Description

GNE Antibody (YA2917) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GNE.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 79 kDa
Species Reactivity Database

Entrez Gene: 10020 Human

SwissProt: Q9Y223 Human

Immunogen

A synthetic peptide of human GLCNE

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105287

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GNE Antibody (YA2917)
    Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg) using GNE (HY-P83172) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    GNE is a Bifunctional enzyme that possesses both UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities, and serves as the initiator of the biosynthetic pathway leading to the production of N-acetylneuraminic acid (NeuAc), a critical precursor in the synthesis of sialic acids. By catalyzing this pivotal and rate-limiting step in sialic acid biosynthesis, this enzyme assumes a pivotal role in governing the regulation of cell surface sialylation, playing a role in embryonic angiogenesis. Sialic acids represent a category of negatively charged sugars that reside on the surface of cells as terminal components of glycoconjugates and mediate important functions in various cellular processes, including cell adhesion, signal transduction, and cellular recognition[1][2][3][4][5][6].

  • Subcellular Localization

    Cytoplasm, cytosol

  • Expression


    Tissue_specificity:Highest expression in liver and placenta. Also found in heart, brain, lung, kidney, skeletal muscle and pancreas. Isoform 1 is expressed in heart, brain, kidney, liver, placenta, lung, spleen, pancreas, skeletal muscle and colon. Isoform 2 is expressed mainly in placenta, but also in brain, kidney, liver, lung, pancreas and colon. Isoform 3 is expressed at low level in kidney, liver, placenta and colon

  • Isoforms & Post-Translational Modification

    Q9Y223 has 5 isomers: Q9Y223-1: 79275 Da (predicted); Q9Y223-2: 83066 Da (predicted); Q9Y223-3: 78579 Da (predicted); Q9Y223-4: 71278 Da (predicted); Q9Y223-5: 66784 Da (predicted).
    Phosphorylated. Phosphorylation by PKC activates the UDP-N-acetylglucosamine 2-epimerase activity

  • Subunit

    Homodimer (PubMed:19841673, PubMed:22343627). Homotetramer (PubMed:26980148). Homohexamer (PubMed:19841673). The hexameric form exhibits both enzyme activities, whereas the dimeric form only catalyzes the phosphorylation of N-acyl-D-mannosamine (By similarity)

  • SwissProt ID

    Q9Y223

  • Gene ID
    10020 [NCBI]
  • Synonyms

    NM; DMRV; IBM2; Uae1; GLCNE

  • Research Field

    Cardiovascular

GNE Antibody (YA2917) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg) using GNE (HY-P83172) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GNE Antibody (YA2917) Product Image
    1/16
  • Experimental Validation Results for GNE Antibody (YA2917)
  • GNE Antibody (YA2917) Product Image
  • Experimental Validation Results for GNE Antibody (YA2917)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg) using GNE (HY-P83172) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for GNE Antibody (YA2917)
  • GNE Antibody (YA2917) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 2(20μg) and K562(lane 3(40μg) using GNE (HY-P83172) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • GNE Antibody (YA2917) Product Image WB