PCGF6 Antibody
(Synonyms: MBLR, RNF134, PCGF6, Polycomb group RING finger protein 6, Mel18 and Bmi1-like RING finger, RING finger protein 134)PCGF6 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PCGF6.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:50-100 |
Product Details
PCGF6 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PCGF6.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 30; 39 kDa
Purified recombinant protein of human PCGF6
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PCGF6 is a Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity[1][2][3].
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Subcellular Localization
Nucleus
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Expression
Tissue_Specificity: Ubiquitous. -
Isoforms & Post-Translational Modification
PCGF6 has 2 isoforms, Q9BYE7-1: amino acid length is 350, molecular weight is 39047 Da (predicted); Q9BYE7-3: amino acid length is 275, molecular weight is 30249 Da (predicted).在有丝分裂期间发生磷酸化。体外实验中，第 30 位丝氨酸(Ser-30)可被 CDK7 磷酸化PCGF6 存在 2 个异构体，Q9BYE7-1：氨基酸个数为 350 个，分子量为 39047 Da (预测)；Q9BYE7-3：氨基酸个数为 275 个，分子量为 30249 Da (预测)。Phosphorylated during mitosis. Phosphorylated on Ser-30 by CDK7 in vitro
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Subunit
Component of a PRC1-like complex.
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SwissProt ID
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Synonyms
MBLR, RNF134, PCGF6, Polycomb group RING finger protein 6, Mel18 and Bmi1-like RING finger, RING finger protein 134
Documentation
[1]. Akasaka T, et al. MBLR, a new RING finger protein resembling mammalian Polycomb gene products, is regulated by cell cycle-dependent phosphorylation. Genes Cells. 2002 Aug;7(8):835-50. [Content Brief]
[2]. Lee MG, et al. Physical and functional association of a trimethyl H3K4 demethylase and Ring6a/MBLR, a polycomb-like protein. Cell. 2007 Mar 9;128(5):877-87. [Content Brief]
[3]. Taherbhoy AM, et al. BMI1-RING1B is an autoinhibited RING E3 ubiquitin ligase. Nat Commun. 2015 Jul 7;6:7621. [Content Brief]
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