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USP5 Antibody (YA6828)

(Synonyms: Deubiquitinating enzyme 5 antibody; Isopeptidase T antibody; ISOT 1 antibody; ISOT antibody; Ubiquitin carboxyl-terminal hydrolase 5 antibody; Ubiquitin isopeptidase T antibody; Ubiquitin specific peptidase 5 (isopeptidase T) antibody; Ubiquitin specific peptidase 5 antibody; Ubiquitin specific processing protease 5 antibody; Ubiquitin specific protease 5 (isopeptidase T) antibody; Deubiquitinating enzyme 5 antibody; Isopeptidase T antibody; ISOT 1 antibody; ISOT antibody; Ubiquitin carboxyl-terminal hydrolase 5 antibody; Ubiquitin isopeptidase T antibody; Ubiquitin specific peptidase 5 (isopeptidase T) antibody; Ubiquitin specific peptidase 5 antibody; Ubiquitin specific processing protease 5 antibody; Ubiquitin specific protease 5 (isopeptidase T) antibody; Ubiquitin specific protease 5 (ubiquitin isopeptidase T) antibody; Ubiquitin thioesterase 5 antibody; Ubiquitin thiolesterase 5 antibody; Ubiquitin-specific-processing protease 5 antibody; UBP5_HUMAN antibody; Usp5 antibody; )
Customer Review

Based on 1 Customer Validation

USP5 Antibody (YA6828) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to USP5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for USP5 Antibody (YA6828)
  • USP5 Antibody (YA6828) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000 1:50 1:200 1:1000

Product Details

Description

USP5 Antibody (YA6828) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to USP5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 96 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 96 kDa
Immunogen

Recombinant protein within Human USP5 aa 759-858 / 858.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for USP5 Antibody (YA6828)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using USP5 Antibody (HY-P87135) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    USP5 is a Deubiquitinating enzyme that participates in a wide range of cellular processes by specifically cleaving isopeptide bonds between ubiquitin and substrate proteins or ubiquitin itself. Affects thereby important cellular signaling pathways such as NF-kappa-B, Wnt/beta-catenin, and cytokine production by regulating ubiquitin-dependent protein degradation. Participates in the activation of the Wnt signaling pathway by promoting FOXM1 deubiquitination and stabilization that induces the recruitment of beta-catenin to Wnt target gene promoter. Regulates the assembly and disassembly of heat-induced stress granules by mediating the hydrolysis of unanchored ubiquitin chains. Promotes lipopolysaccharide-induced apoptosis and inflammatory response by stabilizing the TXNIP protein. Affects T-cell biology by stabilizing the inhibitory receptor on T-cells PDC1. Acts as a negative regulator of autophagy by regulating ULK1 at both protein and mRNA levels. Acts also as a negative regulator of type I interferon production by simultaneously removing both 'Lys-48'-linked unanchored and 'Lys-63'-linked anchored polyubiquitin chains on the transcription factor IRF3. Modulates the stability of DNA mismatch repair protein MLH1 and counteracts the effect of the ubiquitin ligase UBR4. Upon activation by insulin, it gets phosphorylated through mTORC1-mediated phosphorylation to enhance YTHDF1 stability by removing 'Lys-11'-linked polyubiquitination. May also deubiquitinate other substrates such as the calcium channel CACNA1H[1][2][3][4][5][6][7][8].

  • Subcellular Localization

    Cytoplasm,Cytoplasm, Stress granule,Nucleus

  • Isoforms & Post-Translational Modification

    P45974 has two isomers: P45974-1: 95786 Da (predicted); P45974-2: 93308 Da (predicted).
    Ubiquitinated by SMURF1; leading to proteasomal degradation丨SUMOylated at Lys-113; SUMOylation affects the interaction with Cav3

  • Subunit

    Homodimer (PubMed:39900921)

  • SwissProt ID

    P45974

  • Gene ID
    8078 [NCBI]
  • Synonyms

    Deubiquitinating enzyme 5 antibody; Isopeptidase T antibody; ISOT 1 antibody; ISOT antibody; Ubiquitin carboxyl-terminal hydrolase 5 antibody; Ubiquitin isopeptidase T antibody; Ubiquitin specific peptidase 5 (isopeptidase T) antibody; Ubiquitin specific peptidase 5 antibody; Ubiquitin specific processing protease 5 antibody; Ubiquitin specific protease 5 (isopeptidase T) antibody; Deubiquitinating enzyme 5 antibody; Isopeptidase T antibody; ISOT 1 antibody; ISOT antibody; Ubiquitin carboxyl-terminal hydrolase 5 antibody; Ubiquitin isopeptidase T antibody; Ubiquitin specific peptidase 5 (isopeptidase T) antibody; Ubiquitin specific peptidase 5 antibody; Ubiquitin specific processing protease 5 antibody; Ubiquitin specific protease 5 (isopeptidase T) antibody; Ubiquitin specific protease 5 (ubiquitin isopeptidase T) antibody; Ubiquitin thioesterase 5 antibody; Ubiquitin thiolesterase 5 antibody; Ubiquitin-specific-processing protease 5 antibody; UBP5_HUMAN antibody; Usp5 antibody;

USP5 Antibody (YA6828) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using USP5 Antibody (HY-P87135) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • USP5 Antibody (YA6828) Product Image
    1/16
  • Experimental Validation Results for USP5 Antibody (YA6828)
  • USP5 Antibody (YA6828) Product Image
  • Experimental Validation Results for USP5 Antibody (YA6828)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using USP5 Antibody (HY-P87135) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for USP5 Antibody (YA6828)
  • USP5 Antibody (YA6828) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、A549 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using USP5 Antibody (HY-P87135) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • USP5 Antibody (YA6828) Product Image WB