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Phospho-PDHA1 (Ser293) Antibody (YA9899)

(Synonyms: mitochondrial; ODPA_HUMAN; PDH; PDHA; PDHA1; PDHCE1A; PDHE1 A type I; PDHE1-A type I; PHE1A; Pyruvate Dehydrogenase (lipoamide) alpha 1; Pyruvate dehydrogenase complex; E1 alpha polypeptide 1; Pyruvate Dehydrogenase E1 alpha; Pyruvate dehydrogenase E1 com)
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Based on 1 Customer Validation

Phospho-PDHA1 (Ser293) Antibody (YA9899) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-PDHA1 (Ser293).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-PDHA1 (Ser293) Antibody (YA9899)
  • Phospho-PDHA1 (Ser293) Antibody (YA9899) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000 1:500-2000 1:100

Product Details

Description

Phospho-PDHA1 (Ser293) Antibody (YA9899) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Phospho-PDHA1 (Ser293).

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 43 kDa
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser293 of human PDHA1 protein.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-PDHA1 (Ser293) Antibody (YA9899)
    Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg) and Jurkat (lane4, 20μg) using Phospho-PDHA1 (Ser293) Antibody (HY-P810637). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    PDHA1 is a Together with PDHB forms the heterotetrameric E1 subunit of the pyruvate dehydrogenase (PDH) complex. The PDH complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links cytoplasmic glycolysis and the mitochondrial tricarboxylic acid (TCA) cycle. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and dihydrolipoamide dehydrogenase (E3) (Probable). The E1 subunit catalyzes both the thiamine pyrophosphate (TPP)-dependent decarboxylation of pyruvate and the reductive acetylation of a lipoyl group covalently linked to the lipoyl-bearing domains of E2[1][2][3].

  • Subcellular Localization

    Mitochondrion matrix

  • Expression


    Tissue_Specificity: Ubiquitous.

  • Isoforms & Post-Translational Modification

    Phospho-PDHA1 has 4 isoforms, P08559-1: amino acid length is 390, molecular weight is 43296 Da (predicted); P08559-2: amino acid length is 397, molecular weight is 44109 Da (predicted); P08559-3: amino acid length is 359, molecular weight is 40188 Da (predicted); P08559-4: amino acid length is 428, molecular weight is 47580 Da (predicted).
    Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient. Dephosphorylation at all three sites, i.e. at Ser-232, Ser-293 and Ser-300, is required for reactivation.

  • Subunit

    Heterotetramer of two PDHA1 and two PDHB subunits.

  • SwissProt ID

    P08559

  • Gene ID
    5160 [NCBI]
  • Synonyms

    mitochondrial; ODPA_HUMAN; PDH; PDHA; PDHA1; PDHCE1A; PDHE1 A type I; PDHE1-A type I; PHE1A; Pyruvate Dehydrogenase (lipoamide) alpha 1; Pyruvate dehydrogenase complex; E1 alpha polypeptide 1; Pyruvate Dehydrogenase E1 alpha; Pyruvate dehydrogenase E1 com

References

[1]. Seifert F, et al. Phosphorylation of serine 264 impedes active site accessibility in the E1 component of the human pyruvate dehydrogenase multienzyme complex. Biochemistry. 2007 May 29;46(21):6277-87. [Content Brief]

[2]. Kato M, et al. Structural basis for inactivation of the human pyruvate dehydrogenase complex by phosphorylation: role of disordered phosphorylation loops. Structure. 2008 Dec 10;16(12):1849-59. [Content Brief]

[3]. Korotchkina LG, et al. Mutagenesis studies of the phosphorylation sites of recombinant human pyruvate dehydrogenase. Site-specific regulation. J Biol Chem. 1995 Jun 16;270(24):14297-304. [Content Brief]

Phospho-PDHA1 (Ser293) Antibody (YA9899) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg) and Jurkat (lane4, 20μg) using Phospho-PDHA1 (Ser293) Antibody (HY-P810637). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Phospho-PDHA1 (Ser293) Antibody (YA9899) Product Image
    1/16
  • Experimental Validation Results for Phospho-PDHA1 (Ser293) Antibody (YA9899)
  • Phospho-PDHA1 (Ser293) Antibody (YA9899) Product Image
  • Experimental Validation Results for Phospho-PDHA1 (Ser293) Antibody (YA9899)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg) and Jurkat (lane4, 20μg) using Phospho-PDHA1 (Ser293) Antibody (HY-P810637). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Phospho-PDHA1 (Ser293) Antibody (YA9899)
  • Phospho-PDHA1 (Ser293) Antibody (YA9899) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2, 20μg), A549 (lane3, 20μg) and Jurkat (lane4, 20μg) using Phospho-PDHA1 (Ser293) Antibody (HY-P810637). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Phospho-PDHA1 (Ser293) Antibody (YA9899) Product Image WB