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  5. BID Antibody (YA583)

BID Antibody (YA583) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BID.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

  • References

Description

BID Antibody (YA583) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to BID.
Host

Rabbit
Clonality

Monoclonal,Recombinant
Molecular Weight
Predicted band size: 22kDa;
Observed band size: 22kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human BID. The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-2000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:100-500
IHC-F
IHC-F: Immunohistochemistry-Frozen
1:100-500
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
1:100-500
FC
FC: Flow Cytometry
1:50-100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-200
IP
IP: Immunoprecipitation
1:20-50
Sensitivity Endogenous Purity affinity purified by Protein A
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Induces caspases and apoptosis (PubMed:14583606). Counters the protective effect of BCL2 (By similarity); Induces caspase activation and apoptosis (PubMed:15661737, PubMed:32029622). Allows the release of cytochrome c (PubMed:32029622); Induces ICE-like proteases and apoptosis; Induces ICE-like proteases and apoptosis; Does not induce apoptosis; Induces ICE-like proteases and apoptosis
Subcellular Localization：Cytoplasm; Mitochondrion membrane; Mitochondrion outer membrane; Mitochondrion membrane; Mitochondrion membrane; Cytoplasm; Cytoplasm; Mitochondrion membrane
Expression：
Tissue_specificity:It is expressed in the spleen, pancreas, and placenta (protein level) ; it is expressed in the lungs, pancreas, and spleen (protein level) ; it is expressed in the lungs and pancreas (protein level) .
Isoforms & Post-Translational Modification：P55957 has 4 isomers: P55957-1: 21995 Da (predicted); P55957-2: 26836 Da (predicted); P55957-3: 14620 Da (predicted); P55957-4: 11263 Da (predicted).
TNF-alpha induces caspase-mediated cleavage into a major p15 and minor p13 and p11 products (By similarity). Cleaved by CASP6 into a major p15 and minor p13 products, leading to release of cytochrome c and subsequent nonalcoholic steatohepatitis (PubMed:32029622);Ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation
Subunit：Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2 (By similarity). Interacts with PLEKHN1 (PubMed:29531808)
Database

Entrez Gene: 637 Human

SwissProt: P55957 Human

OMIM: 601997 Human

Documentation
User Guide for Antibodies (1077 KB)
References

BID Antibody (YA583) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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BID Antibody (YA583)
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HY-P80031
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