Lactate Dehydrogenase A Antibody (YA317)
(Synonyms: LDHA; LDH-M; LDH1; PIG19; GSD11; TRG5; Proliferation inducing gene 19)Based on 1 Customer Validation
Lactate Dehydrogenase A Antibody (YA317) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Lactate Dehydrogenase A.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-F, IHC-P, ICC/IF, IP
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Reactivity :
Human, Rat
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Formulation:
Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
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Conjugation:
Non-conjugated
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:50-1:100 | 1:50-1:200 | 1:20 |
Product Details
Lactate Dehydrogenase A Antibody (YA317) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Lactate Dehydrogenase A.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Rat
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Observed Molecular WeightObserved band size: 37 kDaNote: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 37 kDa
Entrez Gene: 3939 Human ; 3945 Human ; 16828 Mouse ; 16832 Mouse ; 24533 Rat 24534
SwissProt: P00338 Human ; P07195 Human ; P07864 Human ; P06151 Mouse ; P16125 Mouse ; P04642 Rat P42123
OMIM: 612933 Human
Synthetic peptide corresponding to Human Lactate Dehydrogenase.The exact sequence is proprietary to MCE.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from RAW264.7(lane 2(20ug),C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Lactate Dehydrogenase A Antibody (HY-P80738) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
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Western blot analysis of extracts from Jurkat(lane 2(20ug) , C6(lane 3(20ug) and Hela(lane 4(20ug) using Lactate Dehydrogenase A Antibody (HY-P80738) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded rat liver tissue using Lactate Dehydrogenase A Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded rat liver tissue using Lactate Dehydrogenase A Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunocytochemistry analysis of Hela cells labeling Lactate Dehydrogenase A with Lactate Dehydrogenase A Antibody (HY-P80738)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lactate Dehydrogenase A Antibody (HY-P80738) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
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Immunocytochemistry analysis of NIH/3T3 cells labeling Lactate Dehydrogenase A with Lactate Dehydrogenase A Antibody (HY-P80738) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lactate Dehydrogenase A Antibody (HY-P80738) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Background
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Function
Lactate Dehydrogenase A is an Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+)
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Subcellular Localization
Cytoplasm
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Expression
Tissue_specificity:It is mainly expressed in anaerobic tissues such as skeletal muscle and liver. -
Isoforms & Post-Translational Modification
P00338 has 5 isomers: P00338-1: 36689 Da (predicted); P00338-2: 30057 Da (predicted); P00338-3: 39837 Da (predicted); P00338-4: 30205 Da (predicted); P00338-5: 26712 Da (predicted).
ISGylated -
Subunit
Homotetramer (PubMed:11276087). Interacts with PTEN upstream reading frame protein MP31 (PubMed:33406399). Interacts with folliculin FLCN; the interaction is direct and inhibits enzymatic activity (PubMed:34381247)
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SwissProt ID
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Synonyms
LDHA; LDH-M; LDH1; PIG19; GSD11; TRG5; Proliferation inducing gene 19
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Research Field
Signal Transduction
Documentation
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Data Sheet (234 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)