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Hexokinase I Antibody (YA739)

(Synonyms: HK1; Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I)
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Based on 1 Customer Validation

Hexokinase I Antibody (YA739) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Hexokinase I.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Hexokinase I Antibody (YA739)
  • Hexokinase I Antibody (YA739) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Hexokinase I Antibody (YA739) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Hexokinase I.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 102 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 102 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Hexokinase 1.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102672

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Hexokinase I Antibody (YA739)
    Western blot analysis of extracts from MCF-7 (lane2(20μg), U-87MG (lane3(20μg), C2C12 (lane4(20μg) and Rat brain tissue (lane5(20μg) using Hexokinase I Antibody (HY-P80702). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Hexokinase I catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively). Does not phosphorylate N-acetyl-D-glucosamine. Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan. When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome[1][2][3].

  • Subcellular Localization

    Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasm, cytosol

  • Expression


    Tissue_specificity:Isotype 2: Erythrocyte specific (Reference 6) . Isotype 3: Testis specific (PubMed: 10978502) . Isotype 4: Testis specific (PubMed: 10978502) .

  • Isoforms & Post-Translational Modification

    P19367 has 4 isomers: P19367-1: 102486 Da (predicted); P19367-2: 102201 Da (predicted); P19367-3: 102738 Da (predicted); P19367-4: 101085 Da (predicted).

  • Subunit

    Monomer (PubMed:10686099). Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity). Interacts with VDAC1 (PubMed:22304920). The HK1-VDAC1 complex interacts with ATF2 (PubMed:22304920). Interacts (via N-terminal spermatogenic cell-specific region) with PFKM (via C-terminus) (By similarity). Interacts with SMAD5 (PubMed:28675158)

  • SwissProt ID

    P19367

  • Gene ID
    3098 [NCBI]
  • Synonyms

    HK1; Hexokinase-1; Brain form hexokinase; Hexokinase type I; HK I

  • Research Field

    Cardiovascular

[1]. Magnani M, et al. A recombinant human 'mini'-hexokinase is catalytically active and regulated by hexose 6-phosphates. Biochem J. 1992 Jul 1;285 ( Pt 1)(Pt 1):193-9. [Content Brief]

[2]. Wang F, et al. A missense mutation in HK1 leads to autosomal dominant retinitis pigmentosa. Invest Ophthalmol Vis Sci. 2014 Oct 14;55(11):7159-64. [Content Brief]

[3]. Wolf AJ, et al. Hexokinase Is an Innate Immune Receptor for the Detection of Bacterial Peptidoglycan. Cell. 2016 Jul 28;166(3):624-636. [Content Brief]

Hexokinase I Antibody (YA739) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane2(20μg), U-87MG (lane3(20μg), C2C12 (lane4(20μg) and Rat brain tissue (lane5(20μg) using Hexokinase I Antibody (HY-P80702). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • Hexokinase I Antibody (YA739) Product Image
    1/16
  • Experimental Validation Results for Hexokinase I Antibody (YA739)
  • Hexokinase I Antibody (YA739) Product Image
  • Experimental Validation Results for Hexokinase I Antibody (YA739)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane2(20μg), U-87MG (lane3(20μg), C2C12 (lane4(20μg) and Rat brain tissue (lane5(20μg) using Hexokinase I Antibody (HY-P80702). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Hexokinase I Antibody (YA739)
  • Hexokinase I Antibody (YA739) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7 (lane2(20μg), U-87MG (lane3(20μg), C2C12 (lane4(20μg) and Rat brain tissue (lane5(20μg) using Hexokinase I Antibody (HY-P80702). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • Hexokinase I Antibody (YA739) Product Image WB