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ENSA Antibody (YA2770)

(Synonyms: ARPP-19e)
Customer Review

Based on 1 Customer Validation

ENSA Antibody (YA2770) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ENSA.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for ENSA Antibody (YA2770)
  • Experimental Validation Results for ENSA Antibody (YA2770)
  • Experimental Validation Results for ENSA Antibody (YA2770)
  • ENSA Antibody (YA2770) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:5000 1:50-1:100

Product Details

Description

ENSA Antibody (YA2770) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ENSA.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 13 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 13 kDa
Species Reactivity Database
Immunogen

A synthetic peptide of human ENSA

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ENSA Antibody (YA2770)
    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), U251MG (lane 2, 15 μg), 3T3 (lane 3, 15 μg), and Rat brain (lane 4, 15 μg) using ENSA Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ENSA Antibody (YA2770)
    Western blot analysis of extracts from C6 (lane 2(20μg), NIH/3T3 (lane 3(20μg), Hela (lane 4(20μg), MCF-7 (lane 5(20μg) using ENSA Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ENSA Antibody (YA2770)
    Flow cytometric analysis of 1X106 K562 cells labeling ENSA Antibody (HY-P83025, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    ENSA is a Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase. Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas

  • Isoforms & Post-Translational Modification

    O43768 has 9 isomers: O43768-1: 13389 Da (predicted); O43768-2: 12976 Da (predicted); O43768-3: 15281 Da (predicted); O43768-4: 15534 Da (predicted); O43768-5: 12818 Da (predicted); O43768-6: 12404 Da (predicted); O43768-7: 14710 Da (predicted); O43768-8: 11990 Da (predicted); O43768-9: 14868 Da (predicted).
    Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA

  • Subunit

    Interacts (when phosphorylated at Ser-67) with PPP2R2D (By similarity). Interacts with ABCC8. Interacts with SNCA; interaction is disrupted when phosphorylated at Ser-109.

  • SwissProt ID

    O43768

  • Gene ID
    2029 [NCBI]
  • Synonyms

    ARPP-19e

  • Research Field

    Neuroscience

ENSA Antibody (YA2770) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), U251MG (lane 2, 15 μg), 3T3 (lane 3, 15 μg), and Rat brain (lane 4, 15 μg) using ENSA Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), NIH/3T3 (lane 3(20μg), Hela (lane 4(20μg), MCF-7 (lane 5(20μg) using ENSA Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling ENSA Antibody (HY-P83025, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ENSA Antibody (YA2770) Product Image
    1/16
  • Experimental Validation Results for ENSA Antibody (YA2770)
  • Experimental Validation Results for ENSA Antibody (YA2770)
  • Experimental Validation Results for ENSA Antibody (YA2770)
  • ENSA Antibody (YA2770) Product Image
  • Experimental Validation Results for ENSA Antibody (YA2770)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), U251MG (lane 2, 15 μg), 3T3 (lane 3, 15 μg), and Rat brain (lane 4, 15 μg) using ENSA Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for ENSA Antibody (YA2770)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2(20μg), NIH/3T3 (lane 3(20μg), Hela (lane 4(20μg), MCF-7 (lane 5(20μg) using ENSA Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ENSA Antibody (YA2770)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 K562 cells labeling ENSA Antibody (HY-P83025, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for ENSA Antibody (YA2770)
  • Experimental Validation Results for ENSA Antibody (YA2770)
  • Experimental Validation Results for ENSA Antibody (YA2770)
  • ENSA Antibody (YA2770) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), U251MG (lane 2, 15 μg), 3T3 (lane 3, 15 μg), and Rat brain (lane 4, 15 μg) using ENSA Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight. The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin(HRP), HY-P80993, 1:5000 dilution) was incubated in 5% non-fat milk in TBST for 1 hour at 37°C. Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • ENSA Antibody (YA2770) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6 (lane 2(20μg), NIH/3T3 (lane 3(20μg), Hela (lane 4(20μg), MCF-7 (lane 5(20μg) using ENSA Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • ENSA Antibody (YA2770) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 K562 cells labeling ENSA Antibody (HY-P83025, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ENSA Antibody (YA2770) Product Image WB
  • ENSA Antibody (YA2770) Product Image WB
  • ENSA Antibody (YA2770) Product Image FC