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Importin 9 Antibody (YA7433)

(Synonyms: IMP9, KIAA1192, RANBP9, HSPC273, IPO9, Importin-9, Imp9, Ran-binding protein 9, RanBP9)
Customer Review

Based on 1 Customer Validation

Importin 9 Antibody (YA7433) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Importin 9.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG,Kappa

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Importin 9 Antibody (YA7433)
  • Importin 9 Antibody (YA7433) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000

Product Details

Description

Importin 9 Antibody (YA7433) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Importin 9.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 115 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 115 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG,Kappa

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Importin 9 Antibody (YA7433)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and Mouse testis (lane 4(20μg)) using Importin 9 Antibody (HY-P87748) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Importin 9 is a Nuclear transport receptor that mediates nuclear import of proteins, such as histones, proteasome and actin. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the import of pre-assembled proteasomes into the nucleus; AKIRIN2 acts as a molecular bridge between IPO9 and the proteasome complex. Mediates the nuclear import of histones H2A, H2B, H4 and H4. In addition to nuclear import, also acts as a chaperone for histones by preventing inappropriate non-nucleosomal interactions. Mediates the nuclear import of actin[1][2][3].

  • Subcellular Localization

    Cytoplasm,Nucleus

  • Isoforms & Post-Translational Modification

    Q96P70: 1041 amino acids, molecular weight 115963 Da.

  • Subunit

    Interacts with histones H2A, H2B, H3 and H4 (PubMed:11823430, PubMed:30855230)

  • SwissProt ID

    Q96P70

  • Synonyms

    IMP9, KIAA1192, RANBP9, HSPC273, IPO9, Importin-9, Imp9, Ran-binding protein 9, RanBP9

Importin 9 Antibody (YA7433) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and Mouse testis (lane 4(20μg)) using Importin 9 Antibody (HY-P87748) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Importin 9 Antibody (YA7433) Product Image
    1/16
  • Experimental Validation Results for Importin 9 Antibody (YA7433)
  • Importin 9 Antibody (YA7433) Product Image
  • Experimental Validation Results for Importin 9 Antibody (YA7433)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and Mouse testis (lane 4(20μg)) using Importin 9 Antibody (HY-P87748) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Importin 9 Antibody (YA7433)
  • Importin 9 Antibody (YA7433) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HEK293 (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and Mouse testis (lane 4(20μg)) using Importin 9 Antibody (HY-P87748) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Importin 9 Antibody (YA7433) Product Image WB