THOC3 Antibody (YA8768)

Cat. No.: HY-P89084: Data Sheet User Guide for Antibodies
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THOC3 Antibody (YA8768) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to THOC3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

THOC3 Antibody (YA8768) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to THOC3.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 38.6 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q96J01

Immunogen

Human recombinant protein fragment corresponding to amino acids 15-237 of human THOC3 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-5000

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). Required for efficient export of polyadenylated RNA and spliced mRNA (PubMed:23222130). The THOC1-THOC2-THOC3 core complex alone is sufficient to bind export factor NXF1-NXT1 and promote ATPase activity of DDX39B (PubMed:33191911). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed:15833825, PubMed:15998806, PubMed:17190602)|(Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production

Subcellular Localization：Nucleus,Nucleus speckle

Isoforms & Post-Translational Modification：Q96J01 has two isomers: Q96J01-1: 38772 Da (predicted); Q96J01-2: 36032 Da (predicted).

Subunit：Component of the THO subcomplex, which is composed of THOC1, THOC2, THOC3, THOC5, THOC6 and THOC7 (PubMed:15833825, PubMed:15998806, PubMed:33191911, PubMed:37020021)

Synonyms

hTREX45; THO3

Documentation

User Guide for Antibodies (1077 KB)