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Cat. No.: HY-P83003

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DNAJA1 Antibody (YA2748)

Name: DNAJA1 Antibody (YA2748)
Brand: MedChemExpress
SKU: HY-P83003
Price: 146.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: DNAJA1; DNAJ2; HDJ2; HSJ2; HSPF4; DnaJ homolog subfamily A member 1; DnaJ protein homolog 2; HSDJ; Heat shock 40 kDa protein 4; Heat shock protein J2; HSJ-2; Human DnaJ protein 2; hDj-2)

Customer Review

Based on 1 Customer Validation

DNAJA1 Antibody (YA2748) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DNAJA1.

For research use only. We do not sell to patients.

Host:
Rabbit
Isotype:
IgG
Application:
WB, FC, IP
Reactivity :
Human, Mouse, Rat

Formulation:
Supplied in rabbit IgG in PBS(pH 7.4), 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Conjugation:
Non-conjugated

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications
Product Details
Product Properties
- Appearance
- Formulation
- Concentration
- Storage & Stability
- Shipping
Background
Documentation
References
Help & FAQs
Classifications
Bioz

Applications

Application	WB WB: Western Blot	FC FC: Flow Cytometry	IP IP: Immunoprecipitation
Dilution Ratio	1:1000-1:2000	1:50-1:100	1:50

Product Details

Description

DNAJA1 Antibody (YA2748) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DNAJA1.

Host Rabbit
Clonality Recombinant,Monoclonal
Species Reactivity
Human, Mouse, Rat
Observed Molecular Weight

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 45 kDa

Species Reactivity Database

Entrez Gene: 3301 Human ; 15502 Mouse ; 65028 Rat

SwissProt: P31689 Human ; P63037 Mouse ; P63036 Rat

OMIM: 602837 Human

Immunogen

A synthesized peptide derived from human DNAJA1 aa350-397/397.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

Appearance

Solution
Formulation

Supplied in rabbit IgG in PBS(pH 7.4), 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Concentration

Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Background

Function

DNAJA1 is a Co-chaperone for HSPA8/Hsc70. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro). Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as a co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV^[1]^[2]^[3]^[4].
Subcellular Localization

Membrane; Lipid-anchor; Cytoplasm; Microsome; Nucleus; Cytoplasm, perinuclear region; Mitochondrion
Expression

Tissue_specificity:Ubiquitous. Isoform 2 is highly expressed in testis and lung, but detected at low levels in thymus, prostate, colon and liver

Induction:Up-regulated by heat shock
Isoforms & Post-Translational Modification

P31689 has 2 isomers: P31689-1: 44868 Da (predicted); P31689-2: 37045 Da (predicted).
Subunit

Identified in a complex with HSPA1B and BAX (PubMed:14752510). Interacts with RNF207 (PubMed:25281747)
SwissProt ID

P31689
Gene ID

3301 [NCBI]
Synonyms

DNAJA1; DNAJ2; HDJ2; HSJ2; HSPF4; DnaJ homolog subfamily A member 1; DnaJ protein homolog 2; HSDJ; Heat shock 40 kDa protein 4; Heat shock protein J2; HSJ-2; Human DnaJ protein 2; hDj-2
Research Field

-

Documentation

Select Batch:

Data Sheet (261 KB)
SDS (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
User Guide for Antibodies (1077 KB)

References

[1]. Terada K, et al. Human DnaJ homologs dj2 and dj3, and bag-1 are positive cochaperones of hsc70. J Biol Chem. 2000 Aug 11;275(32):24728-34. [Content Brief]

[2]. Rauch JN, et al. Binding of human nucleotide exchange factors to heat shock protein 70 (Hsp70) generates functionally distinct complexes in vitro. J Biol Chem. 2014 Jan 17;289(3):1402-14. [Content Brief]

[3]. Gotoh T, et al. hsp70-DnaJ chaperone pair prevents nitric oxide- and CHOP-induced apoptosis by inhibiting translocation of Bax to mitochondria. Cell Death Differ. 2004 Apr;11(4):390-402. [Content Brief]

[4]. Stark JL, et al. Structure and function of human DnaJ homologue subfamily a member 1 (DNAJA1) and its relationship to pancreatic cancer. Biochemistry. 2014 Mar 4;53(8):1360-72. [Content Brief]