Fibulin 4 Antibody
(Synonyms: FBLN4, UNQ200/PRO226, EFEMP2, EGF-containing fibulin-like extracellular matrix protein 2, Fibulin-4, Protein UPH1, FIBL-4)Fibulin 4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Fibulin 4.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:100-500 |
Product Details
Fibulin 4 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Fibulin 4.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 49 kDa
Synthetic peptide corresponding to the center region of human Fibulin 4.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Fibulin 4 plays a crucial role in elastic fiber formation in tissue, and in the formation of ultrastructural connections between elastic laminae and smooth muscle cells in the aorta, therefore participates in terminal differentiation and maturation of smooth muscle cell (SMC) and in the mechanical properties and wall integrity maintenance of the aorta. In addition, is involved in the control of collagen fibril assembly in tissue throught proteolytic activation of LOX leading to cross- linking of collagen and elastin (By similarity). Also promotes ELN coacervation and participates in the deposition of ELN coacervates on to microfibrils but also regulates ELN cross- linking through LOX interaction. Moreover adheres to the cells through heparin binding in a calcium-dependent manner and regulates vascularlar smooth muscle cells proliferation through angiotensin signaling[1][2][3][4].
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Subcellular Localization
Secreted, extracellular space, extracellular matrix; Secreted, extracellular space, extracellular matrix, basement membrane
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Isoforms & Post-Translational Modification
Fibulin 4 has an amino acid length of 443, molecular weight is 49405 Da.
N-glycosylated; contains mostly complex-type glycans. Not O-glycosylated. -
Subunit
Homodimer; disulfide-linked.
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SwissProt ID
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Synonyms
FBLN4, UNQ200/PRO226, EFEMP2, EGF-containing fibulin-like extracellular matrix protein 2, Fibulin-4, Protein UPH1, FIBL-4
Documentation
[1]. Sasaki T, et al. Functional consequence of fibulin-4 missense mutations associated with vascular and skeletal abnormalities and cutis laxa. Matrix Biol. 2016 Dec;56:132-149. [Content Brief]
[2]. Cirulis JT, et al. Fibrillins, fibulins, and matrix-associated glycoprotein modulate the kinetics and morphology of in vitro self-assembly of a recombinant elastin-like polypeptide. Biochemistry. 2008 Nov 25;47(47):12601-13. [Content Brief]
[3]. Choudhury R, et al. Differential regulation of elastic fiber formation by fibulin-4 and -5. J Biol Chem. 2009 Sep 4;284(36):24553-67. [Content Brief]
[4]. Djokic J, et al. Fibulin-3, -4, and -5 are highly susceptible to proteolysis, interact with cells and heparin, and form multimers. J Biol Chem. 2013 Aug 2;288(31):22821-35. [Content Brief]
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