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Calmodulin Antibody (YA1827)

(Synonyms: CALM; CALM1; CALM2; CALM3; CAM; CAM1; CAM2; CAM3; CAMB; CAMC)
2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

Calmodulin Antibody (YA1827) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Calmodulin.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P FC
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Calmodulin Antibody (YA1827) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50 1:50-1:100

Product Details

Description

Calmodulin Antibody (YA1827) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Calmodulin.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 17 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 17 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Calmodulin aa100-149.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104023

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Calmodulin Antibody (YA1827)
    Western blot analysis was performed on extracts from C6 (lane 1, 15 μg) using Calmodulin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin , HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
    Western blot analysis of extracts from Hela(lane 2(20ug) , RAW264.7(lane 3(20ug) and C6(lane 4(20ug) using Calmodulin Antibody (HY-P82082) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
    Immunocytochemistry analysis of HeLa cells labeling Calmodulin with Calmodulin antibody (HY-P82082) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calmodulin antibody (HY-P82082) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
    Immunocytochemistry analysis of HeLa cells labeling Calmodulin with Calmodulin antibody (HY-P82082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calmodulin antibody (HY-P82082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Calmodulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Calmodulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
    Flow cytometric analysis of 1X10^6 MCF-7 cells labeling Calmodulin Antibody (YA1827) (HY-P82082, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Calmodulin is the highly conserved, calcium-sensitive protein, that acts as the calcium ion sensor in cells, Ca2+-dependently binds to target proteins, and converts Ca2+ concentrations into cellular signals. Calmodulin has four different Ca2+ binding sites named EF-hand for the sensing of Ca2+ signaling with different amplitude, duration, and location. Calmodulin dysfunction could lead to myocardial contractile problems, neuronal damage, or tumor angiogenesis dysregulation, which may cause cardiovascular diseases, neurological diseases, or cancer[1][2][3].

  • Subcellular Localization

    P0DP23: Cytoplasm, cytoskeleton, spindle; Cytoplasm, cytoskeleton, spindle pole; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cell projection, cilium, flagellum
    P0DP24: Cytoplasm, cytoskeleton, spindle; Cytoplasm, cytoskeleton, spindle pole; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
    P0DP25: Cytoplasm, cytoskeleton, spindle; Cytoplasm, cytoskeleton, spindle pole; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

  • Isoforms & Post-Translational Modification

    Human (P0DP23): 149 amino acids, molecular weight 16,838 Da.
    Human (P0DP24): 149 amino acids, molecular weight 16,838 Da.
    Human (P0DP25): 149 amino acids, molecular weight 16,838 Da.
    Ubiquitination results in a strongly decreased activity. Phosphorylation results in a decreased activity.

  • Subunit

    P0DP23: Homotetramer. Interacts with CEP97, CCP110, TTN/titin and SRY.
    P0DP24: Interacts with MYO1C, MYO5A and RRAD. Interacts with MYO10 (By similarity). Interacts with CEP97, CCP110, TTN/titin and SRY. Interacts with USP6; the interaction is calcium dependent.
    P0DP25: Interacts with CEP97, CCP110, TTN/titin and SRY. Interacts with USP6; the interaction is calcium dependent.

  • SwissProt ID

    P0DP23 / P0DP24 / P0DP25

  • Gene ID
    808 [NCBI]
  • Synonyms

    CALM; CALM1; CALM2; CALM3; CAM; CAM1; CAM2; CAM3; CAMB; CAMC

  • Research Field

    Signal Transduction

[1]. Zeng H, et al. Calmodulin and calmodulin-like protein-mediated plant responses to biotic stresses. Plant Cell Environ. 2023 Dec;46(12):3680-3703. [Content Brief]

[2]. Means AR, et al., Calmodulin--an intracellular calcium receptor. Nature. 1980 May 8;285(5760):73-7. doi: 10.1038/285073a0 [Content Brief]

[3]. Kawasaki H, et al., Molecular Dynamics Study of the Changes in Conformation of Calmodulin with Calcium Binding and/or Target Recognition. Sci Rep. 2019 Jul 23;9(1):10688. [Content Brief]

[4]. Crotti L, et al., Clinical presentation of calmodulin mutations: the International Calmodulinopathy Registry. Eur Heart J. 2023 Sep 14;44(35):3357-3370. [Content Brief]

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Calmodulin Antibody (YA1827) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from C6 (lane 1, 15 μg) using Calmodulin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin , HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , RAW264.7(lane 3(20ug) and C6(lane 4(20ug) using Calmodulin Antibody (HY-P82082) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Calmodulin with Calmodulin antibody (HY-P82082) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calmodulin antibody (HY-P82082) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Calmodulin with Calmodulin antibody (HY-P82082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calmodulin antibody (HY-P82082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Calmodulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Calmodulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 MCF-7 cells labeling Calmodulin Antibody (YA1827) (HY-P82082, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Calmodulin Antibody (YA1827) Product Image
    1/16
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Calmodulin Antibody (YA1827) Product Image
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from C6 (lane 1, 15 μg) using Calmodulin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin , HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , RAW264.7(lane 3(20ug) and C6(lane 4(20ug) using Calmodulin Antibody (HY-P82082) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Calmodulin with Calmodulin antibody (HY-P82082) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calmodulin antibody (HY-P82082) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Calmodulin with Calmodulin antibody (HY-P82082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calmodulin antibody (HY-P82082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Calmodulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Calmodulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 MCF-7 cells labeling Calmodulin Antibody (YA1827) (HY-P82082, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Experimental Validation Results for Calmodulin Antibody (YA1827)
  • Calmodulin Antibody (YA1827) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from C6 (lane 1, 15 μg) using Calmodulin Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin , HY-P80438, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • Calmodulin Antibody (YA1827) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) , RAW264.7(lane 3(20ug) and C6(lane 4(20ug) using Calmodulin Antibody (HY-P82082) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Calmodulin Antibody (YA1827) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling Calmodulin with Calmodulin antibody (HY-P82082) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calmodulin antibody (HY-P82082) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Calmodulin Antibody (YA1827) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling Calmodulin with Calmodulin antibody (HY-P82082) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Calmodulin antibody (HY-P82082) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Calmodulin Antibody (YA1827) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Calmodulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Calmodulin Antibody (YA1827) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Calmodulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Calmodulin Antibody (YA1827) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 MCF-7 cells labeling Calmodulin Antibody (YA1827) (HY-P82082, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti- Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Calmodulin Antibody (YA1827) Product Image WB
  • Calmodulin Antibody (YA1827) Product Image WB
  • Calmodulin Antibody (YA1827) Product Image ICC
  • Calmodulin Antibody (YA1827) Product Image ICC
  • Calmodulin Antibody (YA1827) Product Image IHC-P
  • Calmodulin Antibody (YA1827) Product Image IHC-P
  • Calmodulin Antibody (YA1827) Product Image FC