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CD132 Antibody (YA3953)

(Synonyms: IL2RG; P64; CIDX; IMD4; SCIDX; IL-2RG; SCIDX1)
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Based on 1 Customer Validation

CD132 Antibody (YA3953) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD132.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD132 Antibody (YA3953)
  • CD132 Antibody (YA3953) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

CD132 Antibody (YA3953) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CD132.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Immunogen

Purified recombinant fragment of human CD132 (AA: extra 23-262) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718867

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD132 Antibody (YA3953)
    Western blot analysis of extracts from Jurkat (lane2, 20μg), Raji (lane3, 20μg), Daudi (lane4, 20μg), Jurkat (lane5, 30μg), Raji (lane6, 30μg), K562 (lane7, 30μg) and Daudi (lane8, 30μg) using CD132 Antibody (HY-P84256). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    CD132 is a Common subunit for the receptors for a variety of interleukins. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15[1].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell surface

  • Isoforms & Post-Translational Modification

    P31785 has 2 isomers: P31785-1: 42287 Da (predicted); P31785-2: 20088 Da (predicted).

  • Subunit

    The gamma subunit is common to the IL2, IL4, IL7, IL15, IL21 and probably also the IL13 receptors. Interacts with SHB upon interleukin stimulation. Interacts with IL9 (By similarity)

  • SwissProt ID

    P31785

  • Gene ID
    3561 [NCBI]
  • Synonyms

    IL2RG; P64; CIDX; IMD4; SCIDX; IL-2RG; SCIDX1

CD132 Antibody (YA3953) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane2, 20μg), Raji (lane3, 20μg), Daudi (lane4, 20μg), Jurkat (lane5, 30μg), Raji (lane6, 30μg), K562 (lane7, 30μg) and Daudi (lane8, 30μg) using CD132 Antibody (HY-P84256). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • CD132 Antibody (YA3953) Product Image
    1/16
  • Experimental Validation Results for CD132 Antibody (YA3953)
  • CD132 Antibody (YA3953) Product Image
  • Experimental Validation Results for CD132 Antibody (YA3953)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane2, 20μg), Raji (lane3, 20μg), Daudi (lane4, 20μg), Jurkat (lane5, 30μg), Raji (lane6, 30μg), K562 (lane7, 30μg) and Daudi (lane8, 30μg) using CD132 Antibody (HY-P84256). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD132 Antibody (YA3953)
  • CD132 Antibody (YA3953) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane2, 20μg), Raji (lane3, 20μg), Daudi (lane4, 20μg), Jurkat (lane5, 30μg), Raji (lane6, 30μg), K562 (lane7, 30μg) and Daudi (lane8, 30μg) using CD132 Antibody (HY-P84256). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • CD132 Antibody (YA3953) Product Image WB