HMGCR Antibody (YA3518)

Cat. No.: HY-P81197: Data Sheet COA
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HMGCR Antibody (YA3518) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HMGCR.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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HMGCR Antibody (YA3518) Featured Recommendations:

1 Publications Citing Use of MCE HMGCR Antibody (YA3518)

•Microorganisms. 2026 Mar 26;14(4):740. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

HMGCR Antibody (YA3518) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HMGCR.

Host

Rabbit

Clonality

Monoclonal,Recombinant

Molecular Weight

Predicted band size: 97 kDa;

Observed band size: 97 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P04035

Gene ID

3156 [NCBI]

Immunogen

A synthetic peptide of human HMGCR.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
IP IP: Immunoprecipitation	1:10-1:100

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

1.Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA.
2.Supplied in 1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA.
Please refer to the lot-specific COA for specific buffer information.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Caco-2 (lane 1, 20 μg), K562 (lane 2, 20 μg), and HeLa (lane 3, 20 μg) using HMGCR Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Catalyzes the conversion of (3S)-hydroxy-3-methylglutaryl-CoA (HMG-CoA) to mevalonic acid, the rate-limiting step in the synthesis of cholesterol and other isoprenoids, thus plays a critical role in cellular cholesterol homeostasis (PubMed:21357570, PubMed:2991281, PubMed:36745799, PubMed:6995544). HMGCR is the main target of statins, a class of cholesterol-lowering drugs (PubMed:11349148, PubMed:18540668, PubMed:36745799)

Subcellular Localization：Endoplasmic reticulum membrane; Multi-pass membrane protein; Peroxisome membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Ubiquitously expressed with the highest levels in the cerebellum, fetal brain, testis, skin and adrenal gland; Detected in the cerebellum, fetal brain, testis and adrenal gland; Low abundance except in skin, esophagus, and uterine cervix

Isoforms & Post-Translational Modification：P04035 has 3 isomers: P04035-1: 97476 Da (predicted); P04035-2: 92021 Da (predicted); P04035-3: 99752 Da (predicted).
N-glycosylated. Deglycosylated by NGLY1 on release from the endoplasmic reticulum (ER) in a sterol-mediated manner;Undergoes sterol-mediated ubiquitination and ER-associated degradation (ERAD) (PubMed:12535518, PubMed:19458199, PubMed:21778231). Accumulation of sterols in the endoplasmic reticulum (ER) membrane, triggers binding of the reductase to the ER membrane protein INSIG1 or INSIG2 (PubMed:12535518, PubMed:19458199, PubMed:21778231, PubMed:22143767). The INSIG1 binding leads to the recruitment of the ubiquitin ligase, AMFR/gp78, RNF139 or RNF145, initiating ubiquitination of the reductase (PubMed:12535518, PubMed:19458199, PubMed:21778231). The ubiquitinated reductase is then extracted from the ER membrane and delivered to cytosolic 26S proteosomes by a mechanism probably mediated by the ATPase Valosin-containing protein VCP/p97 (PubMed:12535518, PubMed:19458199, PubMed:21778231). The INSIG2-binding leads to the recruitment of the ubiquitin ligase RNF139, initiating ubiquitination of the reductase (PubMed:22143767). Lys-248 is the main site of ubiquitination (PubMed:19458199). Ubiquitination is enhanced by the presence of a geranylgeranylated protein (PubMed:21778231);Phosphorylated. Phosphorylation at Ser-872 reduces the catalytic activity

Subunit：Homotetramer (PubMed:10698924). Homodimer (PubMed:10698924). Interacts (via its SSD) with INSIG1; the interaction, accelerated by sterols, leads to the recruitment of HMGCR to AMFR/gp78 for its ubiquitination by the sterol-mediated ERAD pathway (PubMed:12535518, PubMed:19458199). Interacts with UBIAD1 (PubMed:23169578)

RRID

AB_3103269

Database

Entrez Gene: 3156 Human ; 15357 Mouse ; 25675 Rat

SwissProt: P04035 Human ; Q01237 Mouse ; P51639 Rat

OMIM: 620375 Human

Synonyms

3 hydroxy 3 methylglutaryl CoA reductase antibody; 3 hydroxy 3 methylglutaryl Coenzyme A reductase antibody; 3 hydroxymethylglutaryl CoA reductase antibody; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH) antibody; 3-hydroxy-3-methylglutaryl-coenzyme A reductase antibody; 3H3M antibody; HMDH_HUMAN antibody; HMG CoA reductase antibody; HMG CoAR antibody; HMG-CoA reductase antibody; Hmgcr antibody; Hydroxymethylglutaryl CoA reductase antibody; LDLCQ3 antibody; MGC103269 antibody; Red antibody;

Documentation

Select Batch:

Data Sheet (235 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

HMGCR Antibody (YA3518) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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HMGCR Antibody (YA3518)

HMGCR Antibody (YA3518) Featured Recommendations:

1 Publications Citing Use of MCE HMGCR Antibody (YA3518)

Product Detail

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Background

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HMGCR Antibody (YA3518) Related Classifications

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