ENT1 Antibody (YA9981)
(Synonyms: ENT1, SLC29A1, Equilibrative nucleoside transporter 1, hENT1, Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter, Solute carrier family 29 member 1, Equilibrative NBMPR-sensitive nucleoside transporter, es nucleoside transporter)ENT1 Antibody (YA9981) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to ENT1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human
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Formulation:
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-500 |
Product Details
ENT1 Antibody (YA9981) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to ENT1.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 50 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 50 kDa
Purified recombinant protein of human ENT1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ENT1 is an Uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis. Functions as a Na(+)-independent transporter. Involved in the transport of nucleosides such as adenosine, guanosine, inosine, uridine, thymidine and cytidine. Also transports purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil). Also mediates the uptake of nicotinamide; contributes to maintaining nicotinamide homeostasis but also regulated nicotinamide biological roles. Mediates basolateral nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis barrier (By similarity). Regulates inosine levels in brown adipocytes tissues (BAT) and extracellular inosine levels, which controls BAT-dependent energy expenditure[1][2][3][4][5][6][7][8][9][10][11][12][13].
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Subcellular Localization
Basolateral cell membrane; Apical cell membrane; Cell membrane
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Expression
Tissue_Specificity: Expressed in testis at the blood-testis barrier (at protein level). Detected in erythrocytes (at protein level). Expressed at relatively high levels in cerebral cortex, particularly the frontal and parietal lobes, and the thalamus and basal ganglia (at protein level). In the midbrain expressed at moderate levels, whereas in the other areas of the brainstem, namely medulla and pons, cerebellum and the hippocampus expressed at lower amounts when compared to the other brain regions (at protein level). Expressed in Langerhans cells and lymphocytes in the pancreas (at protein level). Expressed in kidney, in polarized renal epithelial cells. Expressed in adipose tissues. Expressed in placenta. Expressed in small intestine. -
Isoforms & Post-Translational Modification
ENT1 has 2 isoforms, Q99808-1: amino acid length is 456, molecular weight is 50219 Da (predicted); Q99808-2: amino acid length is 535, molecular weight is 58963 Da (predicted).Glycosylated
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Subunit
Identified in a complex with STOM
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SwissProt ID
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Synonyms
ENT1, SLC29A1, Equilibrative nucleoside transporter 1, hENT1, Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter, Solute carrier family 29 member 1, Equilibrative NBMPR-sensitive nucleoside transporter, es nucleoside transporter
Documentation
References
[1]. Ward JL, et al. Kinetic and pharmacological properties of cloned human equilibrative nucleoside transporters, ENT1 and ENT2, stably expressed in nucleoside transporter-deficient PK15 cells. Ent2 exhibits a low affinity for guanosine and cytidine but a high affinity for inosine. J Biol Chem. 2000 Mar 24;275(12):8375-81. [Content Brief]
[2]. Lum PY, et al. Human intestinal es nucleoside transporter: molecular characterization and nucleoside inhibitory profiles. Cancer Chemother Pharmacol. 2000;45(4):273-8. [Content Brief]
[3]. Mangravite LM, et al. Localization of human equilibrative nucleoside transporters, hENT1 and hENT2, in renal epithelial cells. Am J Physiol Renal Physiol. 2003 May;284(5):F902-10. [Content Brief]
[4]. Endres CJ, et al. Mutation of leucine-92 selectively reduces the apparent affinity of inosine, guanosine, NBMPR [S6-(4-nitrobenzyl)-mercaptopurine riboside] and dilazep for the human equilibrative nucleoside transporter, hENT1. Biochem J. 2004 May 15;380(Pt 1):131-7. [Content Brief]
[5]. SenGupta DJ, et al. Glycine 154 of the equilibrative nucleoside transporter, hENT1, is important for nucleoside transport and for conferring sensitivity to the inhibitors nitrobenzylthioinosine, dipyridamole, and dilazep. Biochem Pharmacol. 2004 Feb 1;67(3):453-8. [Content Brief]
[6]. Visser F, et al. Residues 334 and 338 in transmembrane segment 8 of human equilibrative nucleoside transporter 1 are important determinants of inhibitor sensitivity, protein folding, and catalytic turnover. J Biol Chem. 2007 May 11;282(19):14148-57. [Content Brief]
[7]. Yao SY, et al. Nucleobase transport by human equilibrative nucleoside transporter 1 (hENT1). J Biol Chem. 2011 Sep 16;286(37):32552-62. [Content Brief]
[8]. Aseervatham J, et al. The Role of Flexible Loops in Folding, Trafficking and Activity of Equilibrative Nucleoside Transporters. PLoS One. 2015;10(9):e0136779. [Content Brief]
[9]. Huang W, et al. Functional characterization of human equilibrative nucleoside transporter 1. Protein Cell. 2017 Apr;8(4):284-295. [Content Brief]
[10]. Niemann B, et al. Apoptotic brown adipocytes enhance energy expenditure via extracellular inosine. Nature. 2022 Sep;609(7926):361-368. [Content Brief]
[11]. Griffiths M, et al. Cloning of a human nucleoside transporter implicated in the cellular uptake of adenosine and chemotherapeutic drugs. Nat Med. 1997 Jan;3(1):89-93. [Content Brief]
[12]. Wright NJ, et al. Design of an equilibrative nucleoside transporter subtype 1 inhibitor for pain relief. Nat Commun. 2024 Dec 30;15(1):10738. [Content Brief]
[13]. Chen M, et al. SLC29A1 and SLC29A2 are human nicotinamide cell membrane transporters. Nat Commun. 2025 Jan 30;16(1):1181. [Content Brief]
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