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Patched Antibody

(Synonyms: PTCH, PTCH1, Protein patched homolog 1, PTC, PTC1)

Patched Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Patched.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Patched Antibody
  • Patched Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-2000 1:100-200

Product Details

Description

Patched Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Patched.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 160 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 160 kDa
Immunogen

Synthetic peptide corresponding to the N-term region of human Patched.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Patched Antibody
    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), Ramos (lane 3), MCF-7 (lane 4) and 293T (lane 5) using Patched antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Patched is a Negative regulator of the smoothened signaling pathway, which acts as a receptor for hedgehog (SHH, IHH and DHH) morphogens. In absence of hedgehog, acts as an inhibitor of smoothened protein (SMO) by preventing SMO access to cholesterol: mechanistically, acts as a cholesterol transporter, which depeletes cholesterol from the outer leaflet of plasma membrane, thereby preventing cholesterol-binding to the extracellular CRD region of SMO. Hedgehog-binding inhibits PTCH1 cholesterol transporter activity, promoting SMO activation by cholesterol and transduction of the smoothened signaling pathway. Molecular mechanism governing cholesterol transport are still unclear: may move cholesterol from the outer to the inner leaflet of the membrane in exchange for potassium or sodium ion export in the opposite direction. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis[1][2][3][4][5][6][7][8][9][10][11].

  • Subcellular Localization

    Cell membrane; Cell projection, cilium membrane

  • Expression


    Tissue_Specificity: In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.

  • Isoforms & Post-Translational Modification

    Patched has 4 isoforms, Q13635-1: amino acid length is 1447, molecular weight is 160545 Da (predicted); Q13635-2: amino acid length is 1446, molecular weight is 161038 Da (predicted); Q13635-3: amino acid length is 1381, molecular weight is 153853 Da (predicted); Q13635-4: amino acid length is 1296, molecular weight is 144356 Da (predicted).
    In the absence of hedgehog (SHH, IHH and DHH) ligands, ubiquitination by ITCH at Lys-1426 promotes endocytosis and both proteasomal and lysosomal degradation (By similarity). Ubiquitinated by SMURF1 and/or SMURF2 in response to SHH, promoting endocytosis and lysosomal degradation; degradation is essential for PTCH1 clearance from the primary cilium and smoothened pathway activation (By similarity).

  • Subunit

    Homodimer; two PTCH1 molecules engage one hedgehog (SHH, IHH and DHH) molecule.

  • SwissProt ID

    Q13635

  • Gene ID
    5727 [NCBI]
  • Synonyms

    PTCH, PTCH1, Protein patched homolog 1, PTC, PTC1

[1]. Ma G, et al. Indian hedgehog mutations causing brachydactyly type A1 impair Hedgehog signal transduction at multiple levels. Cell Res. 2011 Sep;21(9):1343-57. [Content Brief]

[2]. Gong X, et al. Structural basis for the recognition of Sonic Hedgehog by human Patched1. Science. 2018 Aug 10;361(6402):. [Content Brief]

[3]. Qi X, et al. Structures of human Patched and its complex with native palmitoylated sonic hedgehog. Nature. 2018 Aug;560(7716):128-132. [Content Brief]

[4]. Qi X, et al. Two Patched molecules engage distinct sites on Hedgehog yielding a signaling-competent complex. Science. 2018 Oct 5;362(6410):. [Content Brief]

[5]. Qian H, et al. Inhibition of tetrameric Patched1 by Sonic Hedgehog through an asymmetric paradigm. Nat Commun. 2019 May 24;10(1):2320. [Content Brief]

[6]. Qi C, et al. Structural basis of sterol recognition by human hedgehog receptor PTCH1. Sci Adv. 2019 Sep;5(9):eaaw6490. [Content Brief]

[7]. Ansell TB, et al. The energetics and ion coupling of cholesterol transport through Patched1. Sci Adv. 2023 Aug 25;9(34):eadh1609. [Content Brief]

[8]. Hu Q, et al. Inhibiting hedgehog signal by a patched-1 antibody. Cell Discov. 2025 Mar 25;11(1):29. [Content Brief]

[9]. Carpenter D, et al. Characterization of two patched receptors for the vertebrate hedgehog protein family. Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13630-4. [Content Brief]

[10]. Rudolf AF, et al. The morphogen Sonic hedgehog inhibits its receptor Patched by a pincer grasp mechanism. Nat Chem Biol. 2019 Oct;15(10):975-982. [Content Brief]

[11]. Johnson RL, et al. Human homolog of patched, a candidate gene for the basal cell nevus syndrome. Science. 1996 Jun 14;272(5268):1668-71. [Content Brief]

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Patched Antibody Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), Ramos (lane 3), MCF-7 (lane 4) and 293T (lane 5) using Patched antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Patched Antibody Product Image
    1/16
  • Experimental Validation Results for Patched Antibody
  • Patched Antibody Product Image
  • Experimental Validation Results for Patched Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), Ramos (lane 3), MCF-7 (lane 4) and 293T (lane 5) using Patched antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Patched Antibody
  • Patched Antibody Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HeLa (lane 2), Ramos (lane 3), MCF-7 (lane 4) and 293T (lane 5) using Patched antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Patched Antibody Product Image WB