Phospho Tyrosine Antibody (YA5306)

Cat. No.: HY-P85614: Data Sheet User Guide for Antibodies
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Technical Support

Phospho Tyrosine Antibody (YA5306) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Phospho Tyrosine.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명
References

제품 설명

Phospho Tyrosine Antibody (YA5306) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Phospho Tyrosine.

Host

Mouse

Clonality

Monoclonal

Species Reactivity

Species independent

SwissProt ID

Gene ID

Immunogen

Phospho-tyrosine containing peptides.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Phosphorylated

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Phosphotyrosine is a reversibly modified amino acid in proteins that acts as a strong hapten; antiphosphotyrosine antibodies (α-pTyr) are a straightforward method to detect protein phosphorylation, which can monitor phosphorylation at any tyrosine of a specific protein, but during detection, the identity of the detected proteins needs to be inferred from their size relative to markers, and more exact verification can be done by stripping the blot and reprobing with antibodies specific to potential target proteins. Phosphotyrosine phosphatases (PTPs) remove phosphate from phosphotyrosine and are regulated by mechanisms including inactivation via oxidation or S-nitrosylation of cysteine in their catalytic sites, localization through localization domains, regulation of enzyme turnover by PEST (proline, glutamic acid, serine, threonine-rich) domains, phosphorylation (serine-threonine phosphorylation is usually inhibitory, while the effect of tyrosine phosphorylation depends on the site, and phosphotyrosine can allosterically activate PTPs with SH2 domains)， and inhibition of membrane-bound PTPs by dimerization. It regulates numerous intracellular signaling pathways by mediating protein-protein interactions, with SH2 domains and phosphotyrosine binding (PTB) domains being two classes of phosphotyrosine-binding domains. Since there is no genetic codon for phosphotyrosine, it is difficult to study phosphotyrosine-mediated binding interactions using DNA-encoded libraries, but in vitro phosphorylated phage-displayed peptide libraries can be used to isolate phosphotyrosine-containing peptide ligands for investigating the binding specificities of related domains.

각종 서류

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/nci-h460-cell.html [Content Brief]