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Heme Oxygenase 1 Antibody (YA5399)

(Synonyms: Heme oxygenase 1; HO-1; )
Customer Review

Based on 1 Customer Validation

Heme Oxygenase 1 Antibody (YA5399) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Heme Oxygenase 1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Application:

    IHC-P, WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL IHC-P ICC
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Heme Oxygenase 1 Antibody (YA5399) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
Dilution Ratio 1:50-200 50-200 1:1000-2000

Product Details

Description

Heme Oxygenase 1 Antibody (YA5399) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Heme Oxygenase 1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 28 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Immunogen

Synthesized peptide derived from human HO-1

Purification

affinity chromatography.

Conjugation

Non-conjugated

Modification

Unmodified

RRID

AB_3719035

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoman tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    Immunohistochemical analysis of paraffin-embedded human liver tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    Immunocytochemistry analysis of Hela cells labeling Heme Oxygenase 1 with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    Immunocytochemistry analysis of NIH-3T3 cells labeling Heme Oxygenase 1 with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Heme Oxygenase 1 catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis; (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy; Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron[1][2][3][4].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Single-pass type IV membrane protein; Cytoplasmic side

  • Expression


    Tissue_specificity:The expression level in renal cell carcinoma tissue is higher than that in normal tissue (protein level) .

    Induction:Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA

  • Subunit

    (Microbial infection) Interacts with SARS-CoV-2 ORF3A protein; the interaction promotes ORF3A-induced autophagy but is unlikely to be involved in ORF3A-mediated induction of reticulophagy

  • SwissProt ID

    P09601

  • Gene ID
    3162 [NCBI]
  • Synonyms

    Heme oxygenase 1; HO-1;

Heme Oxygenase 1 Antibody (YA5399) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoman tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Heme Oxygenase 1 with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling Heme Oxygenase 1 with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Heme Oxygenase 1 Antibody (YA5399) Product Image
    1/16
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Heme Oxygenase 1 Antibody (YA5399) Product Image
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoman tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Heme Oxygenase 1 with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of NIH-3T3 cells labeling Heme Oxygenase 1 with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Experimental Validation Results for Heme Oxygenase 1 Antibody (YA5399)
  • Heme Oxygenase 1 Antibody (YA5399) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Heme Oxygenase 1 Antibody (YA5399) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Heme Oxygenase 1 Antibody (YA5399) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Heme Oxygenase 1 Antibody (YA5399) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoman tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Heme Oxygenase 1 Antibody (YA5399) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Heme Oxygenase 1 Antibody (YA5399) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver tissue using Heme Oxygenase 1 Antibody (YA5399). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P85707, 1/75) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Heme Oxygenase 1 Antibody (YA5399) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Heme Oxygenase 1 with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Heme Oxygenase 1 Antibody (YA5399) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of NIH-3T3 cells labeling Heme Oxygenase 1 with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Heme Oxygenase 1 Antibody (HY-P85707) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Heme Oxygenase 1 Antibody (YA5399) Product Image IHC-P
  • Heme Oxygenase 1 Antibody (YA5399) Product Image IHC-P
  • Heme Oxygenase 1 Antibody (YA5399) Product Image IHC-P
  • Heme Oxygenase 1 Antibody (YA5399) Product Image IHC-P
  • Heme Oxygenase 1 Antibody (YA5399) Product Image IHC-P
  • Heme Oxygenase 1 Antibody (YA5399) Product Image IHC-P
  • Heme Oxygenase 1 Antibody (YA5399) Product Image ICC
  • Heme Oxygenase 1 Antibody (YA5399) Product Image ICC