Cytochrome P450 8B1 Antibody
(Synonyms: CYP12, CYP8B1, 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase, 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, CYPVIIIB1, Cytochrome P450 8B1, Sterol 12-alpha-hydroxylase)Cytochrome P450 8B1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Cytochrome P450 8B1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
Cytochrome P450 8B1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Cytochrome P450 8B1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 58 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 58 kDa
Synthetic peptide corresponding to the center region of human Cytochrome P450 8B1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Cytochrome P450 8B1 is a Sterol 12-alpha-hydroxylase involved in primary bile acid biosynthesis by catalyzing the 12alpha-hydroxylation of key intermediates including 7alpha-hydroxycholest-4-en-3-one, 3alpha,7alpha-dihydroxy-5beta-cholestan-26-oate and chenodeoxycholate along the classic pathway. May also hydroxylate 5beta-cholestane-3alpha,7alpha-diol (By similarity). Functions as a monooxygenase, inserting one atom of molecular oxygen into substrates while reducing the second to water, using electrons supplied by NADPH via cytochrome P450 reductase (CPR) (By similarity). Controls biliary balance of cholic acid and chenodeoxycholic acid, thereby regulating intestinal absorption of dietary lipids (By similarity)[1][2][3].
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Subcellular Localization
Endoplasmic reticulum membrane; Microsome membrane
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Expression
Tissue_Specificity: Liver. -
Isoforms & Post-Translational Modification
Cytochrome P450 8B1 has an amino acid length of 501, molecular weight is 58068 Da.
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SwissProt ID
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Synonyms
CYP12, CYP8B1, 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase, 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase, CYPVIIIB1, Cytochrome P450 8B1, Sterol 12-alpha-hydroxylase
Documentation
References
[1]. Gåfvels M, et al. Structure and chromosomal assignment of the sterol 12alpha-hydroxylase gene (CYP8B1) in human and mouse: eukaryotic cytochrome P-450 gene devoid of introns. Genomics. 1999 Mar 1;56(2):184-96. [Content Brief]
[2]. Fan L, et al. Conversion of chenodeoxycholic acid to cholic acid by human CYP8B1. Biol Chem. 2019 Apr 24;400(5):625-628. [Content Brief]
[3]. Wang Y, et al. CYP8B1 Catalyzes 12alpha-Hydroxylation of C(27) Bile Acid: In Vitro Conversion of Dihydroxycoprostanic Acid into Trihydroxycoprostanic Acid. Drug Metab Dispos. 2024 Oct 16;52(11):1234-1243. [Content Brief]
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