ELK1 Antibody (YA4826)

Cat. No.: HY-P85134: Fiche technique User Guide for Antibodies
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ELK1 Antibody (YA4826) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to ELK1.

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Size		Stock
50 μL		Obtenir un devis
100 μL		Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Description

Description

ELK1 Antibody (YA4826) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to ELK1.

Host

Mouse

Clonality

Monoclonal

Masse moléculaire

Predicted band size: 45 kDa;

Species Reactivity

Human

SwissProt ID

P19419

Gene ID

2002 [NCBI]

Immunogen

Purified recombinant fragment of Elk1 expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200

Pureté	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Background

Function：Transcription factor that binds to purine-rich DNA sequences (PubMed:10799319, PubMed:7889942). Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2 (PubMed:1630903). Induces target gene transcription upon JNK and MAPK-signaling pathways stimulation (PubMed:7889942)

Subcellular Localization：Nucleus

Expression：
Tissue_specificity:lungs and testes

Isoforms & Post-Translational Modification：P19419 has 2 isomers: P19419-1: 44888 Da (predicted); P19419-2: 11217 Da (predicted).
Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention;On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1 (By similarity). Phosphorylated by PRP4K at Thr-417; phosphorylation activation ELK1 transcriptional activity (PubMed:10799319)

Subunit：Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity (PubMed:15920481). Interacts with MAD2L2; the interaction is direct and promotes phosphorylation by the kinases MAPK8 and/or MAPK9 (PubMed:17296730). Interacts with POU1F1 (PubMed:26612202)

Synonyms

ELK1; ETS domain-containing protein Elk-1

Documentation

User Guide for Antibodies (1077 KB)