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SAMD9 Antibody (YA1901)

(Synonyms: SAM domain-containing protein 9)
Customer Review

Based on 1 Customer Validation

SAMD9 Antibody (YA1901) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SAMD9.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, FC

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for SAMD9 Antibody (YA1901)
  • Experimental Validation Results for SAMD9 Antibody (YA1901)
  • Experimental Validation Results for SAMD9 Antibody (YA1901)
  • SAMD9 Antibody (YA1901) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-1:5000 1:50-1:100 1:20-1:50

Product Details

Description

SAMD9 Antibody (YA1901) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SAMD9.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 184 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 184 kDa
Species Reactivity Database

Entrez Gene: 54809 Human

SwissProt: Q5K651 Human

Immunogen

A synthetic peptide of human SAMD9 aa 84-189.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SAMD9 Antibody (YA1901)
    Western blot analysis of extracts from A549 (lane2(20μg), Hela(lane3(20μg), A431(lane4(20μg) and MCF-7(lane5(20μg) using SAMD9 Antibody (HY-P82156). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SAMD9 Antibody (YA1901)
    Immunocytochemistry analysis of A431 cells labeling SAMD9 Antibody with SAMD9 Antibody (YA1901) (HY-P82156) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SAMD9 Antibody (YA1901) ((HY-P82156) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SAMD9 Antibody (YA1901)
    Immunocytochemistry analysis of A431 cells labeling SAMD9 Antibody with SAMD9 Antibody (YA1901) (HY-P82156) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SAMD9 Antibody (YA1901) ((HY-P82156) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    SAMD9 is a Double-stranded nucleic acid binding that acts as an antiviral factor by playing an essential role in the formation of cytoplasmic antiviral granules. May play a role in the inflammatory response to tissue injury and the control of extra-osseous calcification, acting as a downstream target of TNF-alpha signaling. Involved in the regulation of EGR1, in coordination with RGL2. May be involved in endosome fusion[1][2].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Widely expressed. Very low levels are detected in skeletal muscle. Not detected in brain. Down-regulated in aggressive fibromatosis, as well as in breast and colon cancers. Up-regulated in fibroblasts from with normophosphatemic tumoral calcinosis (NFTC)

    Induction:Up-regulated by TNF-alpha through p38 MAPKs and NF-kappa-B. Up-regulated by osmotic shock. Induced by IFNG

  • Subunit

    Interacts with RGL2 (PubMed:21160498). Interacts with EEA1 (PubMed:24029230)

  • SwissProt ID

    Q5K651

  • Gene ID
    54809 [NCBI]
  • Synonyms

    SAM domain-containing protein 9

  • Research Field

    Cell Biology

SAMD9 Antibody (YA1901) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane2(20μg), Hela(lane3(20μg), A431(lane4(20μg) and MCF-7(lane5(20μg) using SAMD9 Antibody (HY-P82156). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling SAMD9 Antibody with SAMD9 Antibody (YA1901) (HY-P82156) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SAMD9 Antibody (YA1901) ((HY-P82156) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling SAMD9 Antibody with SAMD9 Antibody (YA1901) (HY-P82156) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SAMD9 Antibody (YA1901) ((HY-P82156) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SAMD9 Antibody (YA1901) Product Image
    1/16
  • Experimental Validation Results for SAMD9 Antibody (YA1901)
  • Experimental Validation Results for SAMD9 Antibody (YA1901)
  • Experimental Validation Results for SAMD9 Antibody (YA1901)
  • SAMD9 Antibody (YA1901) Product Image
  • Experimental Validation Results for SAMD9 Antibody (YA1901)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from A549 (lane2(20μg), Hela(lane3(20μg), A431(lane4(20μg) and MCF-7(lane5(20μg) using SAMD9 Antibody (HY-P82156). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SAMD9 Antibody (YA1901)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling SAMD9 Antibody with SAMD9 Antibody (YA1901) (HY-P82156) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SAMD9 Antibody (YA1901) ((HY-P82156) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SAMD9 Antibody (YA1901)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A431 cells labeling SAMD9 Antibody with SAMD9 Antibody (YA1901) (HY-P82156) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SAMD9 Antibody (YA1901) ((HY-P82156) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for SAMD9 Antibody (YA1901)
  • Experimental Validation Results for SAMD9 Antibody (YA1901)
  • Experimental Validation Results for SAMD9 Antibody (YA1901)
  • SAMD9 Antibody (YA1901) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from A549 (lane2(20μg), Hela(lane3(20μg), A431(lane4(20μg) and MCF-7(lane5(20μg) using SAMD9 Antibody (HY-P82156). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • SAMD9 Antibody (YA1901) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling SAMD9 Antibody with SAMD9 Antibody (YA1901) (HY-P82156) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SAMD9 Antibody (YA1901) ((HY-P82156) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SAMD9 Antibody (YA1901) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A431 cells labeling SAMD9 Antibody with SAMD9 Antibody (YA1901) (HY-P82156) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with SAMD9 Antibody (YA1901) ((HY-P82156) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • SAMD9 Antibody (YA1901) Product Image WB
  • SAMD9 Antibody (YA1901) Product Image ICC
  • SAMD9 Antibody (YA1901) Product Image ICC