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ATP1A2 Antibody (YA2934)

(Synonyms: Atp1a2; FHM2; KIAA0778; MHP2)
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Based on 1 Customer Validation

ATP1A2 Antibody (YA2934) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP1A2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ATP1A2 Antibody (YA2934)
  • ATP1A2 Antibody (YA2934) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

ATP1A2 Antibody (YA2934) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ATP1A2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 102 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 112 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human ATP1A2 aa50-87.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105304

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATP1A2 Antibody (YA2934)
    Western blot analysis of extracts from HeLa (lane 2(20μg), L929 (lane 3(20μg), C6 (lane 4(20μg) and 3T3-L1 (lane 5(20μg) using ATP1A2 Antibody (HY-P83189) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    ATP1A2 is a This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients

  • Subcellular Localization

    Membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein

  • Expression


    Induction:The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit. Interacts with regulatory subunit FXYD1

  • SwissProt ID

    P50993

  • Gene ID
    477 [NCBI]
  • Synonyms

    Atp1a2; FHM2; KIAA0778; MHP2

  • Research Field

    Signal Transduction

ATP1A2 Antibody (YA2934) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 2(20μg), L929 (lane 3(20μg), C6 (lane 4(20μg) and 3T3-L1 (lane 5(20μg) using ATP1A2 Antibody (HY-P83189) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ATP1A2 Antibody (YA2934) Product Image
    1/16
  • Experimental Validation Results for ATP1A2 Antibody (YA2934)
  • ATP1A2 Antibody (YA2934) Product Image
  • Experimental Validation Results for ATP1A2 Antibody (YA2934)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 2(20μg), L929 (lane 3(20μg), C6 (lane 4(20μg) and 3T3-L1 (lane 5(20μg) using ATP1A2 Antibody (HY-P83189) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for ATP1A2 Antibody (YA2934)
  • ATP1A2 Antibody (YA2934) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 2(20μg), L929 (lane 3(20μg), C6 (lane 4(20μg) and 3T3-L1 (lane 5(20μg) using ATP1A2 Antibody (HY-P83189) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • ATP1A2 Antibody (YA2934) Product Image WB