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Collagen VI A1+A2+A3 Antibody (YA2637)

(Synonyms: Alpha 1 (VI) chain (61 AA); COL6A1; COL6A2; COL6A3; Collagen alpha 2(VI) chain; Collagen alpha 3(VI) chain; OPLL; PP3610)
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Based on 1 Customer Validation

Collagen VI A1+A2+A3 Antibody (YA2637) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen VI A1+A2+A3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Collagen VI A1+A2+A3 Antibody (YA2637)
  • Collagen VI A1+A2+A3 Antibody (YA2637) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200

Product Details

Description

Collagen VI A1+A2+A3 Antibody (YA2637) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Collagen VI A1+A2+A3.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 147 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 109 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Collagen VI (P12109) aa15-250.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104984

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Collagen VI A1+A2+A3 Antibody (YA2637)
    Western blot analysis of extracts from C6 (lane2, 40μg) and NIH/3T3 (lane3, 40μg) using Collagen VI A1+A2+A3 Antibody (HY-P82892). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Collagen VI A1+A2+A3 is a P12109: Collagen VI acts as a cell-binding protein
    P12110: Collagen VI acts as a cell-binding protein
    P12111: Collagen VI acts as a cell-binding protein

  • Subcellular Localization

    P12109: Secreted, extracellular space, extracellular matrix
    P12110: Secreted, extracellular space, extracellular matrix; Membrane; Peripheral membrane protein
    P12111: Secreted, extracellular space, extracellular matrix

  • Subunit

    P12109: Trimers composed of three different chains: alpha-1 (VI) , alpha-2 (VI) , and alpha-3 (VI) or alpha-5 (VI) or alpha-6 (VI)
    P12110: Trimers composed of three different chains: alpha-1 (VI) , alpha-2 (VI) , and alpha-3 (VI) or alpha-5 (VI) or alpha-6 (VI). Interacts with CSPG4
    P12111: Trimers composed of three different chains: alpha-1 (VI) , alpha-2 (VI) , and alpha-3 (VI) or alpha-5 (VI) or alpha-6 (VI)

  • SwissProt ID

    P12109 / P12110 / P12111

  • Gene ID
    1291 [NCBI] / 1292 [NCBI] / 1293 [NCBI]
  • Synonyms

    Alpha 1 (VI) chain (61 AA); COL6A1; COL6A2; COL6A3; Collagen alpha 2(VI) chain; Collagen alpha 3(VI) chain; OPLL; PP3610

  • Research Field

    Cell Biology

Collagen VI A1+A2+A3 Antibody (YA2637) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane2, 40μg) and NIH/3T3 (lane3, 40μg) using Collagen VI A1+A2+A3 Antibody (HY-P82892). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Collagen VI A1+A2+A3 Antibody (YA2637) Product Image
    1/16
  • Experimental Validation Results for Collagen VI A1+A2+A3 Antibody (YA2637)
  • Collagen VI A1+A2+A3 Antibody (YA2637) Product Image
  • Experimental Validation Results for Collagen VI A1+A2+A3 Antibody (YA2637)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane2, 40μg) and NIH/3T3 (lane3, 40μg) using Collagen VI A1+A2+A3 Antibody (HY-P82892). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Collagen VI A1+A2+A3 Antibody (YA2637)
  • Collagen VI A1+A2+A3 Antibody (YA2637) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6 (lane2, 40μg) and NIH/3T3 (lane3, 40μg) using Collagen VI A1+A2+A3 Antibody (HY-P82892). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Collagen VI A1+A2+A3 Antibody (YA2637) Product Image WB