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KAT9 Antibody (YA3382)

(Synonyms: Elongation protein 3 homolog; elp3; hELP3; Kat9)
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Based on 1 Customer Validation

KAT9 Antibody (YA3382) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KAT9.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KAT9 Antibody (YA3382)
  • KAT9 Antibody (YA3382) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:2000 1:50-1:200 1:50

Product Details

Description

KAT9 Antibody (YA3382) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KAT9.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 62 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 62 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human KAT9 / Elp3 aa1-547.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KAT9 Antibody (YA3382)
    Western blot analysis of extracts from Hela (lane 2, 20ug), K562 (lane3, 20μg) and Jurkat (lane4, 20μg) using KAT9 Antibody (HY-P83637). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    KAT9 is a Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase. Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation. Required for acetylation of GJA1 in the developing cerebral cortex[1][2].

  • Subcellular Localization

    Cytoplasm; Nucleus; Nucleus; Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:Expressed in the cerebellum and spinal motor neurons

  • Isoforms & Post-Translational Modification

    Q9H9T3 has 4 isomers: Q9H9T3-1: 62259 Da (predicted); Q9H9T3-2: 60648 Da (predicted); Q9H9T3-4: 49405 Da (predicted); Q9H9T3-5: 52202 Da (predicted).
    Tyrosine-phosphorylated; phosphorylation on Tyr-202 does not affect elongator complex integrity or ELP3 protein stability (PubMed:31341009). Also serine/threonine-phosphorylated (PubMed:31341009)

  • Subunit

    Component of the elongator complex which consists of ELP1, ELP2, ELP3, ELP4, ELP5 and ELP6. ELP1, ELP2 and ELP3 form the elongator core complex (PubMed:11714725, PubMed:11818576, PubMed:19185337, PubMed:22854966, PubMed:25960406). Interacts with alpha-tubulin (PubMed:19185337)

  • SwissProt ID

    Q9H9T3

  • Gene ID
    55140 [NCBI]
  • Synonyms

    Elongation protein 3 homolog; elp3; hELP3; Kat9

  • Research Field

    Epigenetics and Nuclear Signaling

KAT9 Antibody (YA3382) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20ug), K562 (lane3, 20μg) and Jurkat (lane4, 20μg) using KAT9 Antibody (HY-P83637). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • KAT9 Antibody (YA3382) Product Image
    1/16
  • Experimental Validation Results for KAT9 Antibody (YA3382)
  • KAT9 Antibody (YA3382) Product Image
  • Experimental Validation Results for KAT9 Antibody (YA3382)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2, 20ug), K562 (lane3, 20μg) and Jurkat (lane4, 20μg) using KAT9 Antibody (HY-P83637). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for KAT9 Antibody (YA3382)
  • KAT9 Antibody (YA3382) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2, 20ug), K562 (lane3, 20μg) and Jurkat (lane4, 20μg) using KAT9 Antibody (HY-P83637). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • KAT9 Antibody (YA3382) Product Image WB